摘要
甲醛与1,3-环己二酮-组胺在弱酸性缓冲介质中反应生成强荧光化合物,由此建立一种测定食品中痕量甲醛的荧光分光光度法.在优化的实验条件下,反应体系的相对荧光强度与甲醛浓度呈良好的线性关系,该方法的线性范围为0.02～1.00μg/mL,检出限为1.8×10-3μg/mL,对0.75μg/mL甲醛平行测定11次,其相对标准偏差为2.0%.该方法用于食品中甲醛的测定,回收率为81.2%～87.5%,与乙酰丙酮法对照,结果基本一致.
It is found that formaldehyde can react with 1,3-Cyclohexanedione-histamine in the subacidity medium and produce hyperfluorescence componds.Under the optimum conditions,its relative fluorescence intensity is linearly related to the concentration of formaldehyde in the range of 0.02～1.00 μg/mL with a detection limit of 1.8×10-3 μg/mL,the relative standard deviation for the determination of 0.75 μg/mL formaldehyde was 2.0%.The method was applied to determination of formaldehyde in food and the recovery is in the range of 81.2%～87.5%.Compared with acetyacetone method,the results were well.
出处
《重庆文理学院学报（自然科学版）》
2011年第4期50-53,共4页
Journal of Chongqing University of Arts and Sciences
基金
重庆市教委科学技术研究项目(KJ071209)
重庆市现代分析化学重点实验室开放基金计划资助项目(201001)
关键词
甲醛
1
3-环己二酮
组胺
荧光光度法
formaldehyde
1
3-cyclohexanedione
histamine
spectrofluorometric
