摘要
目的观察重组人骨形态发生蛋白-2（rhBMP-2）对体外培养的乳鼠雪旺细胞增殖及生长相关蛋白（GAP-43）表达的影响。方法将纯化的雪旺细胞分两组，一组设为对照，另一种加含终质量浓度为5μg／LrhBMP-2的DMEM／F12培养液培养，在培养后0、12、24、36、48、72h分别用噻唑蓝（MTr）比色法检测不同时间点的A值并绘制生长曲线；用BrdU法测定雪旺细胞增殖率；用Western blot法检测GAP-43蛋白的表达水平。结果经含5μg／LrhBMP-2培养液培养的雪旺细胞，在24、36、48h细胞增殖率明显高于对照组，差异有统计学意义（P〈0．05）；实验组中GAP-43在24、36、48h的表达也显著高于对照组（P〈0．05）。结论rhBMP-2有促进雪旺细胞分裂增殖和GAP-43蛋白表达的作用，可能是其促进周围神经再生的重要机制之一。
Objective To investigate the effects of recombinant human bone morphogenetic pro- tein-2 （rhBMP-2） on the proliferation of Schwann cells and expression of growth associated protein-43 in vitro. Methods Purified Schwann cells were cultured under different conditions according to group assign- ment. In the experiment group, Schwann cells were cultured in DMEM/F12 media supplemented with 5 μg/L rhBMP-2, and in the control group the cells were cultured in the same culture media without rhBMP-2. The Schwann cells in the experiment group were subjected to immunohistochemistry, methyl thiazol tetrazolium （MTr） assay, Bromodeoxyuridine/Hoechst 33342 double staining, and Western blotting, respectively. Results The proliferation rate at 24, 36 and 48 h was significantly increased in the experimental group as compared with the control group （ P 〈 0. 05 ）. Western blotting showed GAP43 protein expression was up-regulated at 24, 36 and 48 h in the experimental group as compared with the control group （P 〈0. 05 ）. Conclusion rhBMP-2 can enhance the proliferation of Schwann cells and expression of growth associated protein43, which could contribute to the underlying mechanisms by which BMP promotes peripheral nerve regeneration.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第9期1553-1555,共3页
Chinese Journal of Experimental Surgery
基金
武汉市卫生局科研立项项目[武卫2010]42号
