摘要
目的:通过建立黄芪药材HPLC指纹图谱,评定不同产地黄芪中化学物质的含量特点,为科学评价黄芪的内在质量提供参考。方法:采用RP-HPLC法,梯度洗脱,测定了10批样品,采用聚类分析及相似度的计算对药材进行鉴别,并对方法进行了评价。结果:根据聚类分析结果,将两年生黄芪药材分为3类,建立了黄芪药材的RP-HPLC指纹图谱共有模型,找出了较大的12个共有峰,指认出其中2个峰分别为黄芪甲苷和芒柄花素。结论:本法简便、快捷、重现性好,建立的黄芪指纹图谱相似度好,且区分性强,为黄芪的质量控制及评价提供了依据。
This study aimed to establish High Performance Liquid Chromatography （HPLC） fingerprint of Shanxi Astragalus to evaluate contents of characteristic components in the materials from different areas and to provide a reference for evaluating the quality of Shanxi Astragalus. Ten batches of Shanxi Astragalus samples were analyzed by RP-HPLC under the gradient elution condition. The clustering analysis and semblance evaluation were performed to discriminate the samples. The HPLC fingerprint of Shanxi Astragalus was established. Samples of two-year-old Shanxi Astragalus were grouped into three categories. Twelve peaks were found existing in all samples. And two of them were identified as the peaks of astragaloside IV and fermononetin. It is concluded that the method established for the HPLC fingerprint of Shanxi Astragalus had good reproducibility. The established HPLC fingerprint can be used in the quality control of Astragalus.
出处
《世界科学技术-中医药现代化》
2011年第4期681-687,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
教育部高校自主课题资助(2011JYBZZ-JS090):基于数据挖掘的芪箭合剂对实验性糖尿病大鼠β细胞功能改善的作用成分的辨识研究
负责人:赵慧辉
山西省科技厅山西省国际科技合作项目(2007081032):山西道地中药黄芪指纹图谱研究
负责人:李桂兰
国家自然基金项目(81102730):基于特征选择的芪箭合剂改善糖尿病大鼠胰岛素β细胞功能的效应成分辨识研究
负责人:赵慧辉
