摘要
目的了解氯硝柳胺悬浮剂(SCN)杀灭日本血吸虫尾蚴效果,寻找适宜杀蚴剂量,为制定预防血吸虫感染的防治策略提供依据。方法将SCN配制成氯硝柳胺有效浓度(W/W)分别为1×10-5、0.5×10-5、1×10-6、0.5×10-6、1×10-7、0.5×10-7溶液,测定SCN杀尾蚴的效果;配制氯硝柳胺有效浓度为1.2×10-5、1.2×10-6、1.2×10-7、1.2×10-8、1.2×10-9、1.2×10-10溶液,用接种环取日本血吸虫尾蚴200条,加入上述浓度的药液中,分别于0、10、30min后投入小鼠,感染30 min,感染后饲养45 d,解剖,观察小鼠感染情况;用SCN 0.02 g/m2分别喷入水体表面,1 h后用哨鼠法测定水体感染性。结果尾蚴接触氯硝柳胺有效浓度≥0.5×10-61 min 100%死亡,≥0.5×10-72 min100%死亡,≥1×10-830 min 100%死亡,<0.5×10-860 min全部存活。尾蚴接触氯硝柳胺有效浓度1.2×10-5,10、30min后小鼠无感染;接触1.2×10-6,10 min后小鼠80%感染,虫负荷为3.2条/只,接触30 min后鼠无感染。现场水体用药前做尾蚴感染试验,感染率为33.33%;用SCN 0.02 g/m2喷洒水表面1 h后,哨鼠测定感染率为0。结论 SCN具有杀灭血吸虫尾蚴作用,可用于灭蚴及预防血吸虫病感染。
Objective To investigate the effect of a suspension of niclosamide（SN） on cercariae of Schistosoma japonicum and effective concentrations of that suspension in order to provide scientific evidence for control strategies to prevent acute schistosomiasis.Methods SN was weighed and formulated into solutions with active concentrations of niclosamide of 1×10-5,0.5×10-5,1×10-6,0.5×10-6,1×10-7,and 0.5×10-7 ml/100 ml in the lab,and the effect of SN on cercariae was determined.Solutions with an active concentration of niclosamide of 1.2×10-5,1.2×10-6,1.2×10-7,1.2×10-8,1.2×10-0,and 1.2×10-10 were formulated.Cercariae were counted 0,10 and 30 min after 200 S.japonicum cercariae were transferred to the solutions and 30 min after mice were infected with the cercariae.Forty-five days after infection,the mice were dissected and the state of infection was observed.SN with a concentration of 0.02 g/m2 was sprayed for 1 h on the surface of water in the field,and then the infectivity of water was determined using sentinel mice.Results All cercariae were killed after coming into contact with an active concentration of niclosamide of more than 0.5×10-6 for 1 min,an active concentration of niclosamide of more than 0.5×10-7 for 2 min,or an active concentration of niclosamide of more than 1×10-8 for 30 min.However,all survived after coming into contact with an active concentration of niclosamide of less than 0.5×10-8 for under 60 min.After the cercariae came into contact with niclosamide of 1.2×10-5 for 10 or 30 min,the cercariae failed to infect mice.When the cercariae were exposed to niclosamide of 1.2×10-6 for 10 min,80% of mice were infected,and the worm burden of mice was 3.2 worms per mouse,but 30 min later the rate of infection in mice decreased to 0.Water in the field was infective,with a rate of infection in sentinel mice of 33.33%.After 0.02 g/m2 SN was sprayed on the water surface for 1 h,water failed to infect sentinel mice.Conclusion SN is toxic to S.japonicum cercariae and can be used to kill cercariae and prevent acute schistosome infections.
出处
《中国病原生物学杂志》
CSCD
2011年第8期600-602,共3页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.81071379)
江苏省卫生厅项目(No.X200309)
关键词
氯硝柳胺悬浮剂
血吸虫
日本
尾蚴
急性感染
Suspension of niclosamide
Schistosoma japonicum
cercaria
acute infection
