摘要
目的:建立蜂胶挥发油中高良姜素与白杨素的含量测定方法。方法:采用高效液相色谱法,选用色谱柱为Agilent ZORBAX Extend(150mm×4.6mm,5μm)C18柱;流动相为甲醇-0.15%磷酸溶液(60∶40),流速为1.0mL/min,检测波长为268nm。结果:高良姜素进样量的质量浓度在0.01031～0.16500μg/mL范围内,线性关系良好(r=0.9996),平均回收率为98.79%,RSD=2.78%;白杨素在0.00938～0.15000μg/mL范围内,线性关系良好(r=0.9998);平均回收率为98.73%,RSD=2.89%。结论:该法简便、快速、准确,重现性好,适用于蜂胶挥发油的质量控制。
Objective： To establish the determination method of the volatile oil of propolis and chrysin galangin. Methods： HPLC column used for the Agilent ZORBAX Extend （150 mm×4.6 mm, 5 μm） C18 column; mobile phase of methanol-0.15% phosphoric acid （60：40） and flow rate 1.0 mL/min. The detection wavelength was 268 nm. Results： The sample size galangin mass concentration of 0.010-0.165μg/mL range, a good linear relationship（r=0.999 6）, the average recovery was 98.79%, RSD=2.78%; chrysin at 0.009-0.150μg/mL range and good linearity（r=0.999 8）; the average recovery was 98.73%, RSD=2.89%. Conclusion： The method is simple, rapid, accurate and reproducible, Its is suitable for quality control of propolis essential oil.
出处
《抗感染药学》
2011年第3期188-190,共3页
Anti-infection Pharmacy
