摘要
阿司匹林抵抗是一个重要的临床问题。研究表明,一些非甾体抗炎药,如布洛芬、吲哚美辛等均可以拮抗阿司匹林对血小板COX-1的抑制作用,导致阿司匹林抵抗。这种非甾体抗炎药-阿司匹林相互作用使得关节炎患者难以使用阿司匹林来预防心血管疾病的发作。在本次实验中,我们在体内、外分别研究了咖啡酸对布洛芬、吲哚美辛和阿司匹林之间相互作用的影响。布洛芬和吲哚美辛对花生四烯酸诱导的血小板聚集均具有弱的抑制作用,同时它们均可以显著抑制阿司匹林的抗血小板聚集活性,但是,它们对咖啡酸的抗血小板聚集活性无明显影响。有趣的是,当咖啡酸和布洛芬以及吲哚美辛同时使用时,可以消除它们对阿司匹林活性的拮抗作用,提示咖啡酸可以逆转布洛芬、吲哚美辛和阿司匹林之间相互作用。体内实验也得到类似的结果。综上所述,我们的实验结果提示,伴有关节炎的心血管疾病患者使用阿司匹林时,补充咖啡酸可以降低非甾体抗炎药对阿司匹林活性的拮抗作用,有利于疾病的治疗。
Aspirin resistance remains an important clinical issue. Previous reports have confirmed that both ibuprofen and indomethacin could antagonize aspirin action at platelet COX-1 level. Such an interaction prevented the patients with arthritis from receving aspirin therapy. We observed the effects of caffeic acid on such an ibuprofen or indomethacin-aspirin interaction both in vitro and in vivo. Both ibuprofen and indomethacin showed weak inhibition on AA-induced platelet aggregation, but significantly antagonized aspirin action on platelet aggregation. In contrast, neither ibuprofen nor indomethacin had effect on caffeic acid action on platelet aggregation. Interestingly, the antagonism of ibuprofen and indomethacin on aspirin action was abolished by simultaneouse addition of caffeic acid, suggesting that caffeic acid could reverse ibuprofen or indomethacin-aspirin interaction. We also obtained the same results from in vivo study. Taken together, our results provides novel insight into aspirin resistance by NSA1Ds and suggests that the NSAIDS treated patients might benefit from the supplement of caffeic acid, a widely distributed polypbenols, when receiving aspirin therapy.
出处
《中国天然药物》
SCIE
CAS
CSCD
北大核心
2011年第5期369-373,共5页
基金
supported by the National Natural Science Foundation of China (No. 30973583)
the Natural Science Foundation of Jiangsu Province (BK2008493)~~
