摘要
目的在体外以缺氧无血清诱导大鼠骨髓问充质干细胞（BMSCs）凋亡，探讨丹酚酸B抗缺氧无血清诱导的大鼠BMSCs凋亡作用。方法体外分离、培养大鼠BMSCs，用分别含0．1、l、10、100mg／L丹酚酸B的完全培养液预处理1h后用磷酸盐缓冲液冲洗2次，再加入含0．1、1、10、100mg／L丹酚酸B的不含血清培养液，共4组，然后与凋亡模型组一起缺氧培养6h，正常对照组常规培养6h，采用Hoechst染色及倒置相差、荧光显微镜观察和AnnexinV／PI双染流式细胞仪检测细胞凋亡。结果缺氧无血清可引起大鼠BMSCs明显凋亡，0．1、1、10mg／L丹酚酸B处理组大鼠BMSCs早期凋亡率较模型组显著降低（P〈0．05）；而中晚期凋亡率和凋亡模型组比较无统计学差异。结论0．1、1、10mg／L丹酚酸B可抗缺氧无血清诱导的大鼠BMSCs早期凋亡，从而提高移植干细胞的存活率。
Objective To investigate the effect of SalB on bone marrow-derived mesenchymal stemcells （BMSCs） apoptosis induced by hypoxia and serum deprivation （hypoxiaJSD） in the vitro. Methods BMSCs were cultured in the vitro and randomly divided into control group, hypoxia/ SD group and SalB group. SalB group was composed by four groups and were pretreated by complete medium with 0.1, 1, 10, 100 mg/L SalB for 1 hour. And after that they were washed with phosphate buffer for 2 times, added by IMDM with 0.1, 1, 10, 100 mg/L SalB and cultured with hypoxia/SD group together in the same condition ofhypoxia/SD for 6 hours. The control group was cultured for 6 hours in the condition of aerobic and enough serum. Apoptosis was detected by Hoechst33342 staining with inverted phase contrast, fluorescence microscope and Annexin V/PI dual-color flow cytometry. Results Significant apoptosis of BMSCs was induced by hypoxia/SD in the vitro. The early apoptosis of BMSCs induced by hypoxia/SD was significantly decreased by SalB of 0.1, 1, 10 mg/L （P〈0.05） . Conclusion 0.1, 1, 10 mg/L SalB can decrease the early apoptosis of BMSCs induced by hypoxia/SD.
出处
《国际中医中药杂志》
2011年第9期807-810,共4页
International Journal of Traditional Chinese Medicine
基金
中国博士后科学基金（项目编号：20090450542）
关键词
丹酚酸B
骨髓间充质干细胞
缺氧无血清
凋亡
SalB
Bone marrow-derived Mesenchymal stem cells
Hypoxia/SD
Apoptosis
