摘要
目的:采用高效液相色谱分析贵州产菝葜属及肖菝葜属8个品种中的活性成分落新妇苷,并通过对8个品种间慢性抗炎试验的对比研究,以对品种间进行药效与质量的评价。方法:RP-HPLC测定落新妇苷含量,色谱条件:依利特HypersilODS2色谱柱(5μm,4.6 mm×250 mm),流动相为乙腈-0.1%冰醋酸(21∶79);流速1 mL.min-1;柱温为30℃;检测波长291nm,理论塔板数以落新妇苷计不得低于5 000。结果:结论:落新妇苷在0.003～3.168μg范围内与峰面积呈良好线性关系,加样回收率(n=9)为100.43%,RSD%=1.5%,8个品种中落新妇苷含量为0.005%～4.493%,含量最低者是小叶菝葜与托柄菝葜,均为0.005 1%,而菝葜中落新妇苷的含量仅为0.009 0%,土茯苓中落新妇苷含量最高为4.5%,是2010年版中国药典(一部)中土茯苓药材中落新妇苷最低限量的10倍以上。通过抗炎试验研究,8个品种对小鼠棉球肉芽形成均具有显著抑制作用,其中土茯苓较其他品种抗炎作用好。本实验可为贵州产菝葜属及肖菝葜属新的药用资源开发利用及土茯苓药材的质量控制提供一定的参考依据。
OBJECTIVE To evaluate the quality of astilbin contained in 8 varieties from Smilax L. and Heterosmilax K. in Guizhou province by HPLC. METttODS Astilbin was determined by HPLC, Elite Hypersil ODS2 column （5μm,4.6mm×250mm） was used, the mobile phase was methyl cyanides-0. 1 % glacial acetic acid （21：79）, and delivered at a flow rate of 1.0 mL·min^-1 ;Column temperature was 30 ℃ Detection wavelength was 291 nm. The theoretical plate number of astilbin was not be less than 5 000. RESULTS Determination of astilbin was carried out by HPLC with a linear range from 0. 003 μg to 3. 168 μg. The recovery rate （n = 9） was 100. 43%, RSD% was equal to 1.46%. The content of astilbin was from 0. 005% to 4. 493%, Both S. microphylla and S. discotis contained the lowest content which were 0. 005 1%, while the astilbin is only 0. 009 0% in S. China, and the highest one that was 4. 5% in S. glabra was 10 more times than the minimum limit in the Phar- macopoeia of People＇s Republic of China 2010. Astibin of 8 varieties could significant inhibit the formation of cotton ball granulation in mice by anti-inflammatory experiments. CONCLUSION This research can provide some references for development of new medicinal resources and qualitative control of Smilax and Heterosmilax plants.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第17期1401-1403,共3页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金资助项目(编号:30960503)
贵州省科学技术基金资助项目[编号:黔科合J字(2008)2143号]
