摘要
从鲤鱼肌肉中提取肌原纤维蛋白,以水解度(DH)和邻苯三酚自由基(DPPH)清除率为指标,探讨了木瓜蛋白酶、碱性蛋白酶和胰蛋白酶水解鲤鱼肌原纤维蛋白的酶解效果。研究表明,木瓜蛋白酶是制备鲤鱼肌原纤维蛋白酶解物的优选蛋白酶,通过单因素及正交试验获得了最佳酶解条件:温度55℃、pH7、酶用量1 000 U/g、底物浓度4%、酶解时间2 h;在此优化条件下鲤鱼肌原纤维蛋白酶解物对DPPH清除率为82.73%,表现出较强的抗氧化活性水平。
The enzymolysis effects of papain,alcalase and trypsin was studied respectively on the common carp（Cyprinus carpio） myofibrillar protein,taking the degree of hydrolysis（DH） and the the scavenging rate of DPPH free radical as index.The results of experiment indicated that papain was the optimum hydrolase for preparing enzymatic hydrolysate of common carp myofibrillar protein.The optimal conditions of hydrolysis was： temperature 55 ℃,pH7,adding enzyme 1 000 U/g,concentration of substrate 4 %,and hydrolysis 2 h by single factor test and the orthogonal experiment.In this condition,the scavenging rate of DPPH free radical were 82.73 %,enzymatic hydrolysate of common carp myofibrillar protein showed high antioxidant activity.
出处
《食品研究与开发》
CAS
北大核心
2011年第8期26-30,共5页
Food Research and Development
关键词
鲤鱼
肌原纤维蛋白
酶解产物
抗氧化性
common carp
myofibrillar protein
enzymatic hydrolysate
antioxidation
