摘要
通过腹腔注射的方式研究了微囊藻毒素-LR(MC-LR)对罗非鱼肝脏活性氧自由基含量(ROS)及相关抗氧化酶,如超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响。实验设计了5个剂量组(对照组、50、100、250、500μg·kg-1MC-LR),并在2、12、24、36、84h检测了ROS含量、SOD和CAT酶活性的变化。结果表明,在MC的作用下,肝脏组织能够产生大量的ROS。SOD和CAT这两种抗氧化酶在清除过量ROS的过程中发挥了不同的作用,SOD酶动态变化的过程表现为在36h突然上升后恢复的趋势,而CAT酶活性在24h和84h有两个高峰。实验结果为以抗氧化酶如SOD、CAT作为监测MC-LR引起罗非鱼毒性作用的生物标志物提供了可行性的依据,为进一步从抗氧化系统的角度研究MC对鱼类的毒性作用机制奠定了基础。
This paper firstly focused on the variation of reactive oxygen species（ROS） and antioxidant enzymes,such as superoxide dismutase（SOD） and catalase（CAT）,to tilapia in exposed to microcystin-LR（MC-LR） by intraperitoneal.In this experiment,five treatments（control,50,100,250,500 μg·kg-1 MC-LR） were designed,the value of ROS,SOD,CAT were detected at time 2,12,24,36,84 h.The results showed that surplus ROS were generated in liver in exposed to MC-LR by intraperitoneal.SOD and CAT played a different role in eliminating the surplus ROS,dynamic process of SOD showed that it had a sudden increase in 36 h,and dynamic process of CAT showed that it had two peaks respectively in 24 h and 84 h.The experiment was to certify that antioxidant enzymes could be used as biomarkers in monitoring the toxic effect of tilapia in exposed to MC in liver,due to their dose and time-response effect variation.The experiment makes a progress in studying the toxic mechanism of microcystin in terms of the antioxidant defence system.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2011年第8期1521-1525,共5页
Journal of Agro-Environment Science
基金
现代农业产业技术体系(CARS-49)
