摘要
目的:探讨党参、黄芪、丹参等益气活血药对心梗后心功能不全大鼠心肌细胞RYR2,SERCA2a,PLB mRNA表达的干预作用。方法:SD大鼠42只,冠状动脉前降支结扎术后随机分成3组:假手术组(对照组)、模型组、中药组。模型组和对照组给予生理盐水灌服。中药组给予益气活血中药浸膏灌胃,治疗4周和8周后分别杀死一半大鼠,取出心脏PCR检测RYR2、SERCA2a及PLB mRNA。结果:各组心衰大鼠RYR2、SERCA2a及PLB mRNA的表达较对照组下降,但中药干预4周后中药组RYR2、SERCA2a及PLB mRNA表达较模型组都有明显增高(P<0.05)。中药干预8周后,RYR2、SERCA2a mRNA仍然高于模型组(P<0.05)。结论:党参、黄芪等中药能抑制心梗后心衰大鼠RYR2、SERCA2a及PLB mRNA下降的作用,但缺乏特异性,可能与益气活血药能促进心肌细胞的能量代谢,防止心衰后心肌细胞的凋亡,心室重构有关。
Objective： To explore the effect of invigorating vital energy （YIQI） and promoting -circulation （HouXue） herbs on expression of RYR2, SERCA2a, PLB mRNA in rat myocardial cells. The Yiqi-Huoxue formula includes Dangshen, Huangqi, Danshen and so on. Methods： 42 myocardial infracted （MI） rats were divided into 3 groups： normal group, model group, herbal group. The model group, and normal group were given normal saline; herbal group were given Yiqi and Huoxue herbs. RYR2, SERCA2a, PLB mRNA expression of all group rats myocardial cells were detected after four and eight week＇s medication. Results： The expression of RYR2, SERCA2a and PLB mRNA of all the groups of heart failure rat myocardial cells was decreasing after 4 week of medication, but that of herb group was higher than model group （P〈0.05）. After 8 weeks of herb treatment, RYR2, SERCA2a mRNA was still higher than that of the model group （P〈0.05）. Conclusion： The research results suggested that Yiqi- Huoxue herbs can inhibit the decrease of RYR2, SERCA2a and PLB mRNA in rat myocardial cells tbut not specificity. It may be related to the mechanism of Yiqi-Huoxue formula in promote the energy metabolism of myocardial cells and prevent apoptosis and ventricular remodeling.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第9期1957-1960,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然基金资助项目(No.30701075)~~
