摘要
目的探讨依达拉奉预处理对大鼠局灶性脑缺血-再灌注损伤的保护机制。方法将36只SD大鼠随机分为假手术组、缺血-再灌注组和依达拉奉组。依达拉奉组术前给予60 mg/(kg.d)的依达拉奉灌胃,共3 d。采用大脑中动脉线栓法制备大鼠缺血-再灌注损伤模型。比较各组神经功能缺损评分、脑梗死体积、血清神经元特异性烯醇化酶(NSE)含量及脑组织白介素-1β(IL-1β)和肿瘤坏死因子(TNF-α)含量。结果与缺血-再灌注组比较,依达拉奉组的神经功能缺损评分显著下降,脑梗死灶体积显著缩小,血清NSE含量明显降低(均P<0.01);脑组织IL-1β和TNF-α含量显著降低(P<0.05～0.01)。结论依达拉奉预处理可减少大鼠脑缺血-再灌注损伤后脑组织IL-1β和TNF-α表达,保护脑组织。
Objective To explore the protection mechanism of Edaravone pretreatment in rats with focal cerebral ischemia-reperfusion injury. Methods Thirty-six SD rats were randomly divided into sham operation group, cerebral ischemia-reperfusion group and Edaravone group. Edaravone group was received Edaravone 60 mg/（ kg · d） by gavage for 3 d before operation. Ischemia-reperfusion injury model was made by embolige the middle cerebral artery. The score of neurological defict, cerebral infarction volumes, the contents of serum neuron-specific Enolase （NSE）, brain tissue interleukin-1β （IL-1β）and tumor necrosis factor-α （TNF-α） were compared among each group. Results Compared with ischemic-reperfusion group, the score of neurological defict in Edaravone group was significantly decreased, cerebral infarction volume was significantly reduced, and serum NSE content was significantly decreased （all P 〈0.01 ）. The contants of IL-11β and TNF-α in brain tissue were significantly lower （P 〈 0.05 - 0. 01 ）. Conclusion Edaravone pretreatment can reduce the expression of IL-1β and TNF-αafter cerebral ischemiareperfusion-injusy, and protect brain tissue.
出处
《临床神经病学杂志》
CAS
北大核心
2011年第4期270-272,共3页
Journal of Clinical Neurology
