摘要
目的:观察雄黄体外抗人肝癌BEL-7402细胞作用和作用机制。方法:采用MTS法和细胞克隆实验法观察雄黄对BEL-7402细胞增殖的影响,应用PI/Hoechest33258双染法和流式细胞仪检测细胞凋亡,荧光分光光度计检测细胞内钙和线粒体膜电位。结果:与阴性对照组比,雄黄能剂量依赖性地抑制BEL-7402细胞的生长和克隆形成。雄黄作用后,BEL-7402细胞出现了凋亡形态学改变如染色质浓缩等;流式细胞仪检测显示,雄黄能剂量依赖性地诱导细胞凋亡,其中,225μg/ml雄黄引起BEL-7402细胞凋亡的百分率为(28.6±4.6)%。与阴性对照组比,雄黄作用后,BEL-7402细胞的细胞内钙升高和线粒体膜电位降低。结论:雄黄具有一定的抗肿瘤作用,其作用机制与升高细胞内钙、降低细胞线粒体膜电位并诱导细胞凋亡有关。
Objective：To investigate anticancer effect of realgar on human hepatocarcinoma cell line BEL-7402 in vitro.Methods：MTS assay and colony formation assay were used to elucidate the antiproliferative effect of realgar on BEL-7402 cells.PI/Hoechest33258 double dying and flow cytometer were carried out for cell apoptosis detection.Intercellular calcium concentration and mitochondrial membrane potential were detected using spectrofluorophotometer.Results：Realgar inhibited cell growth and colony formation of BEL-7402 cells in a dose-dependent manner when comparing with control group.Morphological changes chromatin condense of cell apoptosis were observed in realgar treated BEL-7402 cells,and a dose-dependent apoptosis was shown by flow cytometer analysis.Apoptotic percentage of BEL-7402 cells treated with 225 μg/ml realgar was（28.6±4.6）% and higher than that in the control.Furthermore,intercellular calcium was increased and mitochondrial membrane potential was decreased inBEL-7402 cells treated by realgar compared with control.Conclusion：Realgar has anticancer effect,which might be associated with increasing intercellular calcium,decreasing mitochondrial membrane potential and inducing apoptosis.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2011年第8期936-939,共4页
Journal of Chongqing Medical University
