摘要
目的验证清热合剂的微生物限度检查方法。方法测定清热合剂中5种试验菌的回收率;以大肠埃希菌作为控制菌,金黄色葡萄球菌作为阴性对照菌,考察控制菌检查的专属性。结果 5种试验菌的回收率均达到70%以上;对清热合剂中大肠埃希菌的检查,试验组呈阳性,阴性对照组呈阴性。结论清热合剂的微生物限度检查方法可行,能有效控制该制剂质量。
Objective To establish a method of microbial limit test for Qingre mixture.Methods To determinate the recovery rate of five kinds of test organisms in Qingre mixture.To test the specificity of this test with Escherichia coli bacteria as control bacteria and staphylococcus aureus as negative bacteria.Results Checking this product with the conventional method,the recovery rate of the number of bacteria,fungi and yeast was over 70%.Checking this product with bacteria Escherichia coli as control bacteria,the test group showed positive result and the negative group was negative.Conclusion The microbial limit test of Qingre mixture was feasible.Using the above method,the quality of Qingre mixture can be controlled effectually.
基金
辽宁省教育厅高等学校科学研究项目(05L455)
关键词
清热剂
微生物限度检查
验证
Antipyretic agents
Microbial limit test
Validation of method
