摘要
目的:探讨脂多糖(lipppolys accride,LPS)刺激对大脑皮质胶质细胞自噬相关基因Beclin-1蛋白表达的影响。方法:体外分离新生小鼠大脑皮质混合胶质细胞,采用1 μg/ml LPS刺激24h后收集细胞与培养上清。Western blotting检测Beclin-1蛋白表达水平;CCK-8试剂盒检测细胞增殖率。结果:LPS刺激后,大脑皮质混合胶质细胞Beclin-1蛋白表达水平增高,与对照组比较差异具有统计学意义(P<0.05);细胞增殖率下降,与对照组比较差异亦具有统计学意义(P<0.05)。结论:LPS能激活神经胶质细胞自噬相关基因Beclin-1,这种变化参与调节了胶质细胞的存活情况。
Objective：To study the influence of LPS stimulation on expression of Beclin-1 protein.Methods：Cerebral mixed glia were isolated and cultured in vitro.With 1 μg/ml of LPS-stimulation,mixed glia and cultured supernatant were collected.Beclin-1 protein level and cell proliferaion rate were determined by western blotting and CCK-8 kit respectively.Results：With the treatment of LPS,Beclin-1 protein level was enhanced significantly,when compared with the control（P〈0.05）.The cell proliferation rate decreased,and a signifant difference was found as well（P〈0.05）.Conclusion：LPS can induce the activation of autophagy-related Beclin-1 protein,which may be invovled in the modulation of glia’ death.
出处
《中国当代医药》
2011年第24期12-14,共3页
China Modern Medicine
基金
国家自然科学基金项目(81001340
30771988)
广东省医学科研基金项目(B2010211)
汕头市科技计划(编号:2009-70)资助
