摘要
目的：HPLC分析明目地黄丸中芍药苷、丹皮酚的含量。方法：色谱柱：Zorbax C18（4.6×250mm,5μm）,流动相为甲醇（A）-0.1%冰醋酸梯度洗脱（0～5min,15%-15%A;5～15min,15%～20%A;15～30min,20%～60%A;30～50 min,60%～80%A）,检测波长242nm,进样量20μL,柱温为25℃,流速为1.0mL.min-1分析时间为50min。结果：芍药苷、丹皮酚分别在0.28μg～4.46μg（r=0.9996）和0.34μg～5.46μg（r=0.9998）范围内线性关系良好;平均回收率分别为98.75%（RSD=2.54%）,99.78%（RSD=1.12%）。结论：本方法方便、快捷,重复性好,可作为明目地黄丸的质量控制方法。
Objective： The contents of peoniflorin and paeonol in Mingmu-Dihuang pills was determined simultaneously with HPLC.Method： Zorbax C18（4.6×250mm,5μm）was utilized for separation.The mobile phase was methanol（A） and 0.1% glacial acetic acid（0～5min,15%~15% A;5～15min,15%～20% A;15～30min,20%～60% A;30～50 min,60%～80% A）,the detection wavelength was 242nm,the injection volume was 20 μL with a flow-rate of 1.0mL·min-1.The column temperature was25℃.t=50 min.Results： The calibration curves of linear range of peoniflorin and paeonol were 0.28μg～4.46μg（r=0.9996）,0.34μg～5.46μg（r=0.9998） respectively;The average recoveries were98.75%（RSD=2.54%）,99.78%（RSD=1.12%）.Conclusion： This method is convenient and good recovery.It can be used to control the quality of Mingmu-Dihuang pills.
出处
《内蒙古中医药》
2011年第18期48-49,共2页
Inner Mongolia Journal of Traditional Chinese Medicine
