摘要
为探索猪圆环病毒Ⅱ型(PCVⅡ)ORF2基因的高效表达,将ORF2基因整合到酵母表达载体pPICZαC中,构建重组质粒pPICZαC-ORF2,通过SacⅠ酶切线性化,经电穿孔法转到毕赤酵母菌SMD1168。经ZeocinTM抗性筛选得到转化子,通过SDS-PAGE分析和Western blotting鉴定,结果表明衣壳蛋白在酵母菌中成功分泌表达。
In order to research for high-level expression of porcine circovirus type 2（PCVⅡ） ORF2 gene,the ORF2 gene of PCVⅡ was cloned into the genome of the pPICZαC expression vector to construct the recombinant plasmid pPICZαC-ORF2,and the resultant plasmid digested by SacⅠ was intergraded into the genome of P.pastoris expression strain SMD1168 by electroporation.The transformants were selected with zeocin,and the results of SDS-PAGE and Western blotting showed the capsid protein of PCVⅡ was secretory expressed successfully.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第9期80-83,共4页
China Animal Husbandry & Veterinary Medicine
基金
广东现代农业产业技术体系(F10021)
