摘要
目的探讨氨溴索诱导血红素加氧酶1（HO-1）在胃内容物吸人性肺损伤中的抗炎作用机制。方法40只健康Sprague～Dawley大鼠随机分为4组：对照组、损伤组、氨溴索组、锌原卟啉（Znppix）组。采用蛋白免疫印迹法（Westernblot）检测肺组织HO—1蛋白表达；肺泡灌洗液中炎性细胞计数，并使用ELISA法测定肺泡灌洗液中肿瘤坏死因子-α（TNF-α）、白介素1β（IL-1β）、IL-10含量；电镜下观察肺组织超微结构变化。结果氨溴索组HO-1蛋白表达灰度值较对照组及损伤组均升高（P值均〈o．05）。氨溴索组肺泡灌洗液中自细胞及中性粒细胞计数、TNF-α、IL-lβ、IL—1O较损伤组均降低（P值均〈0．05），而Znppix组较氨溴索组均升高（P值均〈0．05）。电镜下氨溴索组肺组织超微结构较损伤组及Znppix组减轻。结论氨溴索可诱导HO-1表达，进-步通过抑制TNF—α、IL-18、IL-10的产生在胃内容物吸入性肺损伤中发挥抗炎保护作用。
Objective To investigate the anti inflammatory roles of heine oxygenase-1 （HO-1） induced by ambroxol in gastric aspiration lung injury. Methods Forty Sprague-Dawley rats were randomly divided into （our groups：control group, injury group, ambroxol group and znppix group. The expression of HO-1 was examined by Western blot. Leucocyte and neutrophil leucocyte in bronehoalveolar lavage fluid were counted. Tumor necrosis factor-α （TNF-α）, interleukin-1β （IL-1β） and IL-10 in bronchoalveolar lavage fluid were examined by enzyme linked immunosorbent assay. The ultrastructure of the pulmonary alveoli and its capillaries were studied by transmissional electromicroscopy. Results Compared with control group and injury group, the gray value of HO-1 in ambroxol group increased （all P 〈0.05）. Compared with injury group, white blood cell and neutrophil leucocyte eounts,TNF-α,IL-1β and IL-10 in bronehoalveolar lavage fluid of ambroxol group decreased （all P 〈0.05）. Compared with ambroxol group,the above indexes in znppix group increased （all P 〈 0.05 ）. Transmissional electromicroscopy showed that the pathological changes of ambroxol group were milder than those of injury group and znppix group. Conclusions Ambroxol may induc.e the expression of HO-1 and has anti-inflammatory effects by restrainingthe releasing of TNF-α,IL-1β,IL- 10 in gastric aspiration lung injury.
出处
《国际呼吸杂志》
2011年第18期1386-1389,共4页
International Journal of Respiration
