摘要
目的探讨对无精子症患者实施睾丸精子经冷冻复苏后行卵胞浆内单精子注射术(ICSI)的临床效果。方法回顾性分析了既往在我中心有冷冻睾丸精子的34个ICSI周期,评估其冷冻精子复苏、卵子受精、卵裂、可移植胚胎、优质胚胎、临床妊娠及其分娩情况。结果 34个拟定复苏冷冻睾丸精子的周期精子复苏均获得成功,受精率为76.9%(249/324),2PN受精率69.7%(226/324),卵裂率98.8%(246/249),可利用胚胎率84.2%(207/246),优质胚胎率46.3%(114/246);所有周期均有胚胎移植;34个周期中行新鲜胚胎移植30个,种植率为34.4%(21/61),临床妊娠17例(临床妊娠率56.7%)包括13例单胎和4例双胎,其中1例单胎妊娠流产(流产率为5.9%)。目前,有14个周期已经出生了18个健康婴儿(10个男婴,8个女婴)未发现先天缺陷儿,另2例单胎继续妊娠中(32+w,29+w)。结论睾丸精子经冷冻后有很好的复苏率,结合ICSI受精可以得到较好的临床结局。
Objective： To evaluate the clinical outcome of ICSI cycles with frozen-thawed testicular sperm for azoospermia patients.Methods： This study retrospectively investigates thirty-four ICSI cycles scheduled with frozen-thawed testicular sperm.Sperm recovery,fertilization,cleavage,embryo quality,pregnancy and implantation rate,pregnancy outcome and the healthy condition of newborn babies were evaluated.Results： All the frozen testicular sperm samples were recovered successfully.The fertilization rate,2PN fertilization rate,cleavage rate,feasible using embryo rate,good quality embryo rate was 76.9%,69.7%,98.8%,84.2%,46.3% respectively.All cycles have embryos to transfer.Fresh Embryos were transferred in thirty cycles of thirty-four cycles.The clinical pregnancy rate and implantation rate was 56.7%（21/61）,34.4%（21/61） respectively,including thirteen singletons and four twins,one miscarriage was happened in early pregnancy period.Up to now,there are fourteen pregnancies resulted in eighteen healthy babies including ten boys and eight girls and two other ongoing pregnancies（32＋w,29＋w）.Conclusions： The clinical outcome of ICSI cycles with frozen-thawed testicular sperm is good for azoospermia patients.
出处
《中国优生与遗传杂志》
2011年第9期110-112,共3页
Chinese Journal of Birth Health & Heredity
基金
北京大学第一医院青年基金资助
关键词
无精子症
ICSI
精子冷冻
睾丸活检
Azoospermia
ICSI
Sperm cryopreservation
Testicular biopsy
