摘要
目的建立液-质联用鉴别中药狼毒药材来源的方法。方法瑞香狼毒、狼毒大戟及月腺大戟药材饮片分别粉碎后用70%乙醇超声提取30 min,提取液经微孔滤膜过滤后,利用液-质联用法进行分析。结果从3种狼毒药材提取物的液相色谱及质谱图中选择18个紫外吸收及质谱响应较好的色谱峰,并对其定性,可作为不同种属狼毒药材鉴别的特征峰。结论该方法简便快速,能够用于狼毒药材饮片来源的鉴别分析。
Objective To establish an LC-MS method to identify the Chinese herbal medicine Langdu.Methods The pieces of the Stellera chamaejasme L.,Euphorbia fischeriana Steud and Euphorbia ebiacteolata Hayata were crashed into floccus by a pulverizer,and extracted with 70% alcohol ultrasound extraction for 30 min,individually.The solution of the extract was filtered through a filter membrane for LC-MS analysis.Results Eighteen peaks with good UV absorption and MS response on LC-MS chromatogram of extracts were selected as the characteristic compound to identify 3 kinds of Langdu.Conclusion The method is simple,quick,and can be used to identify the source of Chinese herbal medicine Langdu.
出处
《中南药学》
CAS
2011年第8期597-600,共4页
Central South Pharmacy
关键词
狼毒
瑞香狼毒
狼毒大戟
月腺大戟
液-质联用
鉴别
Langdu root； Stellera chamaejasme L.； Euphorbia fischeriana Steud； Euphorbia ebiacteolata Hayata； LC-MS； identification；
