摘要
目的:建立同时测定银黄胶囊中黄芩苷和绿原酸含量的测定方法,进行测定条件的考察。方法:紫外分光光度法进行黄芩苷和绿原酸吸收点、提取溶剂、提取时间的考察;高效液相色谱法进行流动相组成、比例的考察。结果:HPLC法同时测定银黄胶囊中黄芩苷和绿原酸的含量经方法学验证,结果良好。结论:该研究为银黄胶囊含量的测定打下了良好的基础,其测定方法简便可行。
Objective：To establish a method to determinate the quantity of baicalin and chlorogenic acid in Yinhuang capsules simultaneously.Methods：The isoabsorptive point of baicalin and chlorogenic acid,the solvent agent and the ex-tracting time were detected by UV,the constitution and rate of mobile phase were determined by HPLC.Results：The quantity of baicalin and chlorogenic acid in Yinhuang capsules was determined simultaneously by HPLC and the method was confirmed methodologically.Conclusion：The method has the advantage of easiness and efficiency,and lays firm foundation for studies on the quantity determination of Yinhuang capsules.
出处
《河南大学学报（医学版）》
CAS
2011年第3期166-169,共4页
Journal of Henan University:Medical Science
基金
河南大学2010年度大学生创新性实验计划校级项目(10NB028)
关键词
银黄胶囊
黄芩苷
绿原酸
HPLC法
条件考察
Yinhuang capsule
Baicalin
Chlorogenic acid
HPLC method
Determination
