摘要
目的验证和评价VITEK2高级专家系统（AES）判定鲍曼不动杆菌产头孢菌素酶AmpC的可靠性。方法首先收集2009年l～8月经VITEK2的AES判定为产AmpC酶鲍曼不动杆菌，用AmpC纸片法进行确证试验，最后用聚合酶链反应验证阳性株是否存在AmpC基因。结果15株AES判断为产AmpC酶的菌株，经AmpC纸片法确证试验为阳性，其中12株阳性产酶株可以检测到AmpC基因。132株AES判断为不产AmpC酶的菌株中，经AmpC纸片法验证均为阴性。结论VITEK2AES可以很好地预测鲍曼不动杆菌产AmpC酶情况，有助于快速、准确地检测鲍曼不动杆菌这一相关耐药机制，为临床治疗多重耐药鲍曼不动杆菌提供更有力的支持。
Objective To evaluate the reliability of Acinetobacter baumannii strains producing cephalosporinase judged by ad- vanced experts system（AES） in VITEK2. Methods Acinetobacter baumannii strains,producing cephalosporinases and judged by AES,were collected from January 2009 to August 2009. Then AmpC disk test was performed to identify cephaiosporinase positive strains. Finally,PCR was performed to amplify ampC gene in AmpC positive strains. Results All AmpC positive strains judged by AES were identified to produce cephalosporinases. PCR showed that 12 positive strains were positive with AmpC gene. 132 AmpC negative strains demonstrated could not produce cephalosporinases by AmpC disk test. Conclusion VITEK2 AES system could ac- curately identify cephalosporinase phenotype of Acinetobacter baumannii so as to provide more helpful data to clinician.
出处
《国际检验医学杂志》
CAS
2011年第14期1533-1534,1537,共3页
International Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(30770935)
广东省卫生厅资助项目(A2009234)
关键词
鲍氏不动杆菌
头孢菌素酶
耐药机制
Acinetobacter baumannii
cephalosporinase~ drug-resistant mechanism
