摘要
目的:探讨不同时期自发性高血压大鼠(SHR)前列腺组织中神经生长因子(NGF)及其mRNA的表达变化及其意义。方法:1月龄(幼年期)、6月龄(成年期)和12月龄(老年期)SHR组和血压正常WKY组各5只,分别计算腹侧前列腺指数;并采用免疫组化和RT-PCR方法,分别检测各组腹侧前列腺组织中NGF及其mRNA表达情况。结果:1月龄时,SHR和WKY前列腺指数分别为:1.16±0.06、1.03±0.09;6月龄时,SHR和WKY前列腺指数分别为:1.12±0.14、0.93±0.07;12月龄时,SHR和WKY前列腺指数分别为:1.11±0.05、0.96±0.09;SHR组腹侧前列腺指数无论成年期还是老年期均高于WKY组(P<0.05),在幼年期则差别不明显(P>0.05)。NGF及其mRNA在SHR组和WKY组的不同时期的腹侧前列腺组织中均有表达,随着月龄增长,NGF及其mRNA在SHR组和WKY组的表达逐渐增强(P<0.05);在同一个月龄期,SHR组表达强度高于WKY组(P<0.05)。结论:在发生BPH的SHR中,其交感神经兴奋性增强可能是BPH和高血压发病的共同机制之一,NGF在这一环节中发挥重要作用。
Objective： To investigate the changes of the expressions of the nerve growth factor（NGF） and its mRNA in the prostate tissues of spontaneously hypertensive rats（SHR） of different ages and their significance.Methods： SHRs and normotensive Wistar Kyoto（WKY） rats were killed at 1 month（young）,6 months（adult） and 12 months（aging）,respectively,5 in each group.Their prostate indexes were calculated,and the expressions of NGF and its mRNA in the ventral prostate tissue were detected by immunohistochemistry and RT-PCR.Results： The prostate indexes of the SHR and WKY groups were 1.16 ± 0.06 and 1.03 ± 0.09 at 1 month,1.12 ± 0.14 and 0.93 ± 0.07 at 6 months,and 1.11 ± 0.05 and 0.96 ± 0.09 at 12 months,significantly higher in the former group than in the latter either at 6 or at 12 months（P0.05）,but with no obvious difference at 1 month（P0.05）.The expressions of NGF and its mRNA in the ventral prostate tissue were detected in all groups,and elevated gradually with the increase of age（P0.05）.Among those of the same age,the expression levels were markedly higher in the SHR than in the WKY group（P0.05）.Conclusion： In SHRs with benign prostate hyperplasia（BPH）,the enhanced excitation of the sympathetic nervous system may be a common mechanism underlying BPH and hypertension,and NGF plays an important role in it.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2011年第9期781-784,共4页
National Journal of Andrology
基金
福建省教育厅科技项目(JGG200715)
关键词
良性前列腺增生
高血压
神经生长因子
自发性高血压大鼠
benign prostate hyperplasia
hypertension
nerve growth factor
spontaneously hypertensive rat
