摘要
从鄱阳湖地区3种耕作层的土壤中分离出17种菌株,经过初筛及复筛获得1株有较强絮凝活性的菌株（CSB3）,经鉴定为革兰式阴性杆菌。采用单因素试验方法和正交试验设计方法,分析了影响絮凝效果的主要因素,对该菌株的最佳培养条件进行了优化研究。结果表明：金属离子Mg2＋对产生菌的絮凝活性有较大影响,Mg2＋更适合做该产生菌的助凝剂。该产生菌的絮凝活性主要集中在上清液中（絮凝率为71.4%）,表明该微生物絮凝剂是微生物在发酵过程中由菌细胞分泌的胞外物质。搅拌速度在80~120 r/min时有较好的絮凝活性。CSB3菌株产絮凝剂的最佳条件为：质量分数为1%（g/mL）Mg2＋的投加量为3 mL,pH为12,搅拌速度为120 r/min,温度为30℃,培养时间为26~28 h。CSB3菌株产生的絮凝剂对质量分数为5‰高岭土悬浊液的最高絮凝率为93.1%。
An optimum strain CSB3 with high flocculating activity was chosen by preliminary screening and rescreening from 17 strains of bacteria,which were isolated from 3 kinds of cultivated soil in the area of Poyang Lake.By preliminary identification,CSB3 was discovered as a rod,gram-negative strain.Then the major factors of impacting flocculation effect were analyzed and the best cultural conditions of CSB3 bacterial strain were studied by single factor test and orthogonal design test.The experimental results showed that metal ion Mg2＋ had a significant effect on flocculation activity from the producing strain,Mg2＋ was more appropriate for flocculation aid to the producing strain.The flocculation activity from the producing strain concentrated mainly on supernatant（flocculation rate 71.4%）,which showed that the microorganism flocculant was an
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2011年第4期817-822,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省科技厅资助项目(2009AE00600
2009BNB06100
200513222)
南昌大学鄱阳湖环境与资源利用教育部重点实验室开发基金资助项目(Z05002)
关键词
微生物絮凝剂
筛选
培养条件
耕作土壤
m icrobial flocculent
screening
culture cond ition
cultivated soil
