摘要
对野西瓜Capparis spinosa L.果实中化学成分进行提取分离,进一步探索野西瓜抗氧化活性成分,为野西瓜的充分开发利用提供科学依据.取野西瓜果实粗粉,采用95%乙醇回流提取,分别用石油醚、氯仿、乙酸乙酯、正丁醇、水萃取3次,反复萃取得各个部位,再用DPPH.清除实验比较各部位抗氧化活性,然后针对所确定抗氧化活性部位,经硅胶柱、凝胶柱分离,结合薄层层析、碘蒸气显色、荧光显色、通用显色剂显色等方法,得到单体化合物.最后,采用DPPH.清除实验测定各单体化合物的抗氧化活性.野西瓜各萃取部位的消除能力大小为:乙酸乙酯部位>水部位>正丁醇部位>氯仿部位>石油醚部位;从野西瓜活性部位-乙酸乙酯部位分离得到3个化合物,即化合物1长寿花糖苷、化合物2琥珀酸、化合物3水苏碱甲酯,其中长寿花糖苷消除能力较强,琥珀酸和水苏碱甲酯的清除效果不明显.乙酸乙酯部位为野西瓜抗氧化活性部位,长寿花糖苷是乙酸乙酯部位的抗氧化活性成分之一.
Chemical compositions in Capparis spinosa L.fruit were extracted and separated,to further explore chemical compositions with antioxidant activity in Capparis spinosa L.fruit.Capparis spinosa L.fruit meal was extracted The petroleum ether,ethyl aetate,butanol and water were extracted repeated respectively.Antioxidant activity of extracts were determined by DPPH·cleaning experiment.Identified antioxidant extract was separated by silica gel column,gel column with TLC,iodine vapor reagent,fluorescenrt color and universal reagent.Antioxidant compounds were got through DPPH·experiment.The eliminating ability of every extact was ethyl acetate〉 water position〉n-butanol〉 chloroform parts〉 petroleum ether.Three compouds were isolated from ethyl acetate extract： 1 kalanchoe glycosides,2 succinate,3 methyl stachydrine.Kalanchoe glycosides showed strong elimination ability while the other two showed low elimination ability.Ethyl acetate extract was the antioxidant active part of Capparis spinosa L.,and kalanchoe glycosides was one of the antioxidant compounds in ethyl acetate extract.
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2011年第4期524-527,共4页
Journal of Harbin University of Commerce：Natural Sciences Edition
基金
国家自然科学基金项目(30902013)
