摘要
探讨莱菔硫烷(sulforaphane,SFN)诱导人肝癌HepG-2细胞凋亡过程中,JNK途径的作用.采用荧光显微镜观察凋亡细胞形态;采用Western Blot法检测人肝癌HepG-2细胞内JNK和p-JNK蛋白的表达.结果表明,10、20、40μmol/L的SFN作用人肝癌HepG-2细胞48 h后,荧光显微镜下可见随着药物浓度的增大,凋亡细胞数量逐渐增多,并呈现典型的凋亡细胞形态;HepG-2细胞内p-JNK蛋白表达量逐渐升高,而对JNK的表达无明显影响.提示SFN可诱导HepG-2细胞的发生凋亡,激活JNK信号通路可能是其主要机制之一.
To investigate the roles of JNK pathway in apoptosis induced by sulforaphane(SFN) in human hapetocelluar carcinoma HepG-2 cells.Fluorescence microscope was used to observe the morphological changes of HepG-2 cells after treated by different dosage of SFN.Western Blot assay was employed to detect the expression of JNK and p-JNK proteins.The results showed that after treated by SFN at the dosage of 10,20,40 μmol/L for 48 h,the apoptotic cell numbers increased rapidly when the dose rose and showed typical apoptotic morphology.The expression of p-JNK protein was evidently up-regulated,meanwhile,there was no remarkable change on the expression of JNK protein.A conclusion can be drawn that SFN can induce cell apoptosis of HepG-2 cells by activating JNK pathway in HepG-2 cells.
出处
《哈尔滨商业大学学报(自然科学版)》
CAS
2011年第4期532-535,共4页
Journal of Harbin University of Commerce:Natural Sciences Edition
基金
国家自然科学基金资助项目(30300284)
黑龙江省自然科学基金项目(D200802)
黑龙江省骨干教师项目(1154G36)