摘要
目的观察C57/BL6小鼠海马发育老化过程中Bcl-2、Bax蛋白的表达变化。方法取不同胚龄(embryonicday,E)、生后(postnatal day,P)日龄或月龄的小鼠海马,应用免疫组化及图像分析技术检测Bcl-2和Bax蛋白的表达。结果 E16 d^P7 d,海马CA区锥体细胞层和齿状回颗粒细胞层Bcl-2和Bax阳性细胞均逐渐增多,平均吸光度(average optical,AO)值逐渐增大;P14 d^P21 d阳性细胞逐渐减少,CA区AO值逐渐减小,齿状回AO值先增大后减小;P28 d后趋于稳定。CA区Bcl-2/Bax比值E16 d^E20 d增大,P1 d减小,P3 d^P5 d增大,P7 d^P21 d减小;以后趋于稳定。齿状回Bcl-2/Bax比值只在P1 d降低。结论 Bcl-2和Bax蛋白可能与小鼠海马发育和老化过程中的形态学变化密切相关。
Objective To observe the changes of expression of Bcl-2 and Bax protein in the development and aging of C57/BL6 mouse hippocampus.Methods Immunohistochemical and image analysis methods were used to detect the expression of Bcl-2 and Bax protein in the mouse hippocampus of different age.Results From embyronic（E） 16 day（d） to postnatal day（P） 7 d,Bcl-2 and Bax immunoreactive cells increased in the pyramidal layer and granular layer,and the average optical（AO） increased too.From P14 d to P21 d,immunoreactive cells decreased,and their AO decreased gradually in the CA area;AO increased first but then decreased in dentate gyrus.On P28 d,the immunoreactive cells and their AO were unchanged.Moreover,the ratio of Bcl-2/Bax rose on E16 d to E20 d,diminished on P1 d,increased on P3 d to P5 d,and decreased on P7 d to P21 d.Then the ratio of Bcl-2/Bax remained unchanged after P28 d.Conclusion Bcl-2 and Bax protein may be correlated with the morphological changes in the development and aging of mouse hippocampus.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2011年第5期553-556,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
