摘要
能够有效富集、分离蛋白质的人工合成材料在蛋白质组学研究中具有重要应用价值。本文利用氨丙基三乙氧基硅烷的氨基与普施安蓝分子中三嗪环上氯原子之间的取代反应生成普施安蓝修饰的烷氧基硅烷,在四乙氧基硅烷存在下,通过自组装溶胶-凝胶法制备了普施安蓝掺杂的二氧化硅纳米棒。扫描电镜表征显示纳米棒的长度在2～16μm之间,直径在200～500 nm之间。红外光谱、热重-差示扫描量热法表征结果表明:普施安蓝被成功掺入二氧化硅纳米棒。所制备纳米棒对牛血清白蛋白的吸附容量达到57.6 mg/g,30 min内达到饱和容量的80%。通过对不同蛋白质的吸附研究表明:其对胰蛋白酶和溶菌酶表现了较好的吸附,吸附量分别为87.5 mg/g和59.8mg/g;而其几乎不吸附牛血红蛋白和胃蛋白酶,尤其是对牛血红蛋白的吸附量只有3 mg/g。以上研究结果表明:所制备纳米棒材料不仅对蛋白质有较高的吸附容量,而且也表现出良好的选择性,该材料有望进一步应用于蛋白质组学中对相关蛋白质的富集与分离。
Protein enrichment and separation is one of the pivotal preliminary steps of proteomics studies,which is important to medical diagnosis and treatment.In this study,procion brilliant blue-doped silica nanorod was prepared via self-assembly sol-gel technology without any additional template.Procion brilliant blue was covalently linked to 3-aminopropyltriethyloxy silane in ethanol.Tetraethylorthosilane（TEOS） was then added into the mixture,subsequently hydrolyzed and co-condensed for 3 h under stirring.The resulted nanorods were isolated by centrifugation,re-dispersed in deionized water,and centrifuged again.This wash process was repeated three times.Finally,the nanorods were dried under vacuum.Procion brilliant blue acted simultaneously as a self-assembly template during the preparation process,and subsequently as recognition probe for proteins.Scanning electron microscopy（SEM） image showed that the nanotubes were 2-16 μm in length and 200-500 nm in diameter.The obtained nanorods were further characterized by Fourier transform infrared spectroscopy（FTIR）,thermogravimetric analysis（TGA） and differential scanning calorimetry（DSA）,separately.All these results indicated that procion brilliant blue were successfully doped into silica nanorods.The recognition property of nanorods for bovine serum albumin（BSA） was investigated under static condition.The resulted nanorods showed high binding capacity（57.6 mg/g） for BSA and fast adsorption equilibrium（within 60 min）.The nanorods were also evaluated with four typical proteins,hemoglobin,trypsin,lysozyme and pepsin,with different relative molecular masses and isoelectric points.The results indicated that the prepared nanorods exhibited the highest binding capacity for trypsin（87.5 mg/g） and the least binding for hemoglobin（Hb,3.0 mg/g）.This easy preparation protocol and excellent recognition property make the prepared materials a bright future in proteomics research.
出处
《色谱》
CAS
CSCD
北大核心
2011年第9期876-880,共5页
Chinese Journal of Chromatography
基金
国家自然科学基金项目(No.21075089)
天津科技大学人才引进基金项目(No.20090424)
