摘要
[目的]研究影响东方白果(Solanum texanum)种子无菌苗萌发的因素,为其组织培养奠定基础。[方法]采取不同的预处理和消毒方法处理种子,观察其萌发情况。[结果]用70℃水浸种10 m in,经75%乙醇消毒5 m in和0.1%HgC l2消毒30 s,放置于灭菌的脱脂棉中培养,可在7 d内获得无菌苗,种子萌发率可达到75.0%。[结论]该方法适合东方白果种子无菌苗的萌发,也可为其他科属种皮坚硬种子的萌发提供依据。
[Objective] This paper was to study germination factors for sterile seedling of Solanum texanum seeds.The results laid the foundation for tissue culture.[Method] The seeds were to deal with different pretreatments and disinfectants,then the germination results were observed.[Result] The seeds were soaked in 70 ℃ water for 10 min,then in 75% ethyl alcohol for 5 min and 0.1% HgCl2 for 30 s respectively.The sterile seedlings were obtained after one week when the seeds were placed in sterile cotton.Seeds germination rate reached 75%.[Conclusion] This method was fit for germination of Solanum texanum seeds and provided basis for germination of other hard seeds.
出处
《安徽农业科学》
CAS
北大核心
2011年第26期15882-15882,15884,共2页
Journal of Anhui Agricultural Sciences
基金
牡丹江师范学院青年专项基金项目(QF200902)
关键词
东方白果
无菌苗
萌发
Solanum texanum
Sterile seedling
Germination
