摘要
[目的]纯化马蹄蕨黄酮并研究其抗氧化活性。[方法]在以70%乙醇为溶剂、料液比1∶40、超声时间1 h、超声功率140 W的条件下提取了马蹄蕨黄酮;以吸附量和解析率为指标研究了分离纯化马蹄蕨黄酮的最佳工艺;并通过测定还原能力、清除DPPH和羟自由基能力研究了所得黄酮的抗氧化活性。[结果]AB-8树脂的纯化效果最好,黄酮纯度达40.6%;马蹄蕨黄酮具有较强的抗氧化活性,一定范围内抗氧化活性和浓度呈正相关。[结论]为马蹄蕨资源的综合开发利用提供了理论依据。
[Objective] The aim was to purify Horseshoe fern flavonoids and study its antioxidant activity.[Method] Flavonoids were extracted from Horseshoe fern under the conditions of ethanol concentration 70%,material/liquid 1∶40,ultrasonic power 140 W and extracting time 1 h.The optimal isolation and purification technology of Horseshoe fern flavonoids was studied by taking adsorption capacity and desorption rate as indexes.Furthermore,the antioxidant activity of orseshoe fern flavonoids was studied by deter mining their clear ability about DPPH radicals and OH radicals and their reductive ability.[Result] The AB-8 macroporous resin showed the best adsorption and desorption ability comparing with the other seven kind of macroporous resins,and the purity of flavonoid reached 40.6%.Total flavonoids of Horseshoe fern had strong antioxidant activity.The antioxidant activity of Horseshoe fern flavonoids was positively correlated with the concentration within a certain range.[Conclusion] The research provides theoretical basis for the comprehensive development and utilization of Horseshoe fern resources.
出处
《安徽农业科学》
CAS
北大核心
2011年第26期15922-15923,15926,共3页
Journal of Anhui Agricultural Sciences
关键词
马蹄蕨
黄酮
纯化
抗氧化性
Horseshoe fern
Flavonoids
Purification
Antioxidant activity
