摘要
目的:观察加味桃核承气汤对β-淀粉样蛋白(Aβ)损伤大鼠学习记忆能力的影响,并探讨其作用机制。方法:SD大鼠随机分成3组,利用立体定位仪将Aβ1-42全长肽注射到大鼠双侧海马,构建Aβ损伤痴呆鼠模型(即Aβ1-42模型组);同时注射Aβ42-1反序列全长肽(即Aβ42-1对照组)和磷酸盐缓冲液(PBS)对照组(PBS对照组)。造模7 d后,每组再分成2个亚组,即加味桃核承气汤治疗组和空白组,分别以灌胃法(ig)给予加味桃核承气汤20 mL(20 g.kg-1)和同样体积的生理盐水,1次/d,连续21 d。利用水迷宫定位航行实验检测各组大鼠学习记忆能力情况,并应用Westen blotting方法检测各组大鼠海马组织中Tau,p-Tau蛋白的表达情况。结果:在定位航行实验中,Aβ1-42模型组大鼠平均逃避潜伏期(59.20±10.12)s长于Aβ42-1对照组(34.26±15.23)s和PBS对照组(32.07±9.50)s(P<0.05);Aβ1-42模型组大鼠应用加味桃核承气汤治疗后,平均逃避潜伏期明显缩短,与给予生理盐水组相比存在统计学差异(P<0.05);而应用加味桃核承气汤治疗后Aβ42-1对照组和PBS对照组的平均逃避潜伏期改变不明显。此外,应用加味桃核承气汤治疗的Aβ1-42模型组大鼠海马组织中Tau和p-Tau蛋白的表达水平也低于生理盐水组。结论:加味桃核承气汤可能通过降低脑组织中Tau蛋白表达,抑制Tau蛋白异常磷酸化,提高痴呆大鼠学习记忆能力。
Objective：To observe the effect of Jiawei Taohe Chengqi decoction（JWTHCQD）on learning and memory in Aβ1-42 induced Alzheimer＇s disease（AD）in rats and study its mechanism.Method：Rats were injected bilaterally of aggregated Aβ1-42,Aβ42-1 or PBS into hippocampus mounted using stereotaxic apparatus for AD model establishment.Three groups rats were treated with JWTHCQD（20 g·kg-1）or saline solution respectively.After 21 days,learning and memory ability of the rats were determined by Morris water maze test.And hippocampus tissue specimens for each group were harvested for Tau and p-Tau protein detcted by Western blotting.Result：In place navigation test,the time of escape latency was significantly longer in Aβ1-42 group than that in other groups（P0.05）,but shorter in JWTHCQD treated Aβ1-42 group（P0.05）.Besides,Tau and p-Tau protein were all down-regulation in the Aβ1-42 group after JWTHCQD treatment.Conclusion：JWTHCQD could improve the learning and memory in AD model rats by regulating Tau and p-Tau protein expression.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第19期174-176,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
教育部博士点基金新教师项目(20092133120001)
