摘要
目的:观察头痛宁胶囊对偏头痛模型大鼠三叉神经节P2X3mRNA表达的影响。方法:健康SD大鼠随机分为:正常对照组(生理盐水组)、模型组(偏头痛模型组)、西比灵组1.04 mg.kg-1、头痛宁高、中、低剂量组(760,380,190 mg.kg-1)。预防给药7 d。第7天ig 30 min后sc硝酸甘油(NTG,10 mg.kg-1)制备偏头痛大鼠模型,造模后3,6 h 2个时间点取材,用RT-PCR方法检测各组大鼠三叉神经节P2X3mRNA的表达情况。结果:与正常对照组比较,模型组大鼠三叉神经节P2X3mRNA表达数增多(P<0.01),3 h多于6 h。与模型组比较,头痛宁高、中、低剂量治疗组和西比灵治疗组大鼠三叉神经节P2X3mRNA表达数均减少(均P<0.01)。结论:头痛宁胶囊可能通过抑制由硝酸甘油诱导的P2X3mRNA过度表达,发挥治疗偏头痛的作用。
Objective：To observe the effects of Toutongning capsule on P2X3mRNA expression in trigeminal ganglion of migraine rats.Method：The healthy SD rats were evenly divided into：normal group.model group,three dose groups of Toutongning（760,380,190 mg·kg-1）capsule group and Xibiling group.Migrained rats model was established by NTG subcutaneous injection.Samples were harvested and measured in 3 hours and 6 hours after NTG subcutaneous injection.The P2X3mRNA expression in trigeminal ganglion was determined by RT-PCR.Result： P2X3mRNA expression in trigeminal ganglion increased in the model group compared with that in the normal group（P0.01）.P2X3mRNA expression was elevated at hour 3 then decreased at hour 6.P2X3mRNA expression in trigeminal ganglion and thalamencephalon were downregulated in the three dose groups of Toutongning capsule and Xibiling group compared with those in the model group（P0.01）.Conclusion： Toutongning capsule could treat migraine by reducing P2X3mRNA expression in trigeminal ganglion.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第19期188-190,共3页
Chinese Journal of Experimental Traditional Medical Formulae
