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HPLC法测定不同产地黑豆皮中矢车菊素-3-O-葡萄糖苷的含量 被引量:12

HPLC Determination of Cyanidin-3-O-Glucoside in Black Soybean Hulls Grown in Different Regions of China
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摘要 目的:建立黑豆皮中矢车菊素-3-O-葡萄糖苷的含量测定方法,测定5个不同产地黑豆皮中矢车菊素-3-O-葡萄糖苷的含量。方法:采用HPLC法测定黑豆皮中矢车菊素-3-O-葡萄糖苷的含量,色谱柱采用Phenomenex LunaSu C18柱(250mm×4.60mm,5μm);流动相A相为0.5%磷酸溶液,B相为水-乙腈(50:50),进行梯度洗脱;流速0.8mL/min;检测波长520nm;柱温30℃;进样量10μL。结果:矢车菊素-3-O-葡萄糖苷标准曲线回归方程为:Y=2×107X-33120(r=0.9998),在0.1041~1.041μg范围内线性关系良好,平均回收率分别为92.4%、92.5%和95.5%,不同产地黑豆皮中矢车菊素-3-O-葡萄糖苷含量范围为5.263~12.829mg/g。结论:所用方法简便、准确,可用于不同产地黑豆皮的质量控制。 Objective: To determine cyanidin-3-O-glucoside(C3G) content in black soybean hulls grown in different regions of China by HPLC.Methods: The chromatographic separation was performed on Phenomenex Luna Su C18(250 mm × 4.60 mm,5 μm) column by gradient elution using 0.5% phosphoric acid solution as mobile phase A and water: acetonitrile(50:50,V/V) as mobile phase B at a flow rate of 0.8 mL/min.The column temperature was set at 30 ℃and the detection wavelength at 520 nm.Results: The developed regression equation of cyanidin-3-O-glucoside standard is: Y = 2 × 107X-33120(r= 0.9998).A good linearity was shown in the range of 0.1041-1.041 μg.The average recoveries at three spike levels were 92.4%,92.5% and 95.5%,respectively.The contents of cyanidin-3-O-glucoside in samples from Northeast China and Shandong,Hubei and Anhui provinces were in the range of 5.263-12.829 mg/g.Conclusions: The method is simple and reliable,and may be used for the quality control of black soybean hull from different geographic origins.
出处 《食品科学》 EI CAS CSCD 北大核心 2011年第18期256-259,共4页 Food Science
基金 天津市科技支撑项目(09ZCKFNC01000)
关键词 高效液相色谱法(HPLC) 黑豆皮 矢车菊素-3-O-葡萄糖苷 含量测定 high performance liquid chromatography(HPLC) black soybean hulls cyanidin-3-O-glucoside content determination
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