摘要
以宁夏引种种植的33份黑核桃树种和1份本地核桃树种为试验材料,采用改进的CTAB法提取高质量模板DNA,通过24条RAPD引物利用RAPD-PCR技术系统分析材料间的遗传关系.结果表明:试验所筛选的18条RAPD引物可以用于黑核桃树种遗传资源鉴定,较为准确地将种间材料聚类;33份黑核桃材料与作为对照参试的普通核桃亲缘关系较远,参试黑核桃材料间则表现出较近的亲缘关系.
33 pieces of Juglans nigra and 1 Juglans were used as experimental materials,the developed CTAB method was used to extract high quality DNA,and RAPD-PCR by 24 primers was employed to analyze the genetics relationship between the experimental materials.The result showed that 18 RAPD primers could be used in appraise Juglans nigra genetics resource and clustering the experimental materials correctly.Genetics relationship date showed that 33 experimental materials of Juglans nigra was farer than Juglans,nevertheless closer genetics relationship among Juglans nigra experimental materials.
出处
《农业科学研究》
2011年第3期27-30,共4页
Journal of Agricultural Sciences
基金
宁夏科技计划资助项目
宁夏林业科学研究项目(宁林研[2010]06号)
