摘要
应用ISSR标记,对96份海南龙血树种质的遗传多样性进行研究。从100个ISSR引物中筛选出10个多态性明显、反应稳定的引物,共扩增出134条谱带,平均每个引物能扩增出13.4条带,多态性条带比率达99.25%。材料间遗传相似系数为0.589 6～0.985 1,POPGENE结果分析表明,平均Shannon信息指数(I)为0.370 2,平均Nei’s基因多样性(H)为0.232 1,每位点平均有效等位基因(NE)为1.369 2。用UPGMA法将96份海南龙血树种质分为4大类。
Ninety-six Dracaena cambodiana Pierre ex Gagnep germplasms were studied for genetic diversity analysis by ISSR with ten effective primers selected from 100 ISSR primers. 133 of 134 DNA fragments were amplified, showed polymorphisms. The average polymorphic bands percentage for the 134 amplified fragments was 99.25%. The coefficient of genetic conformability among the germplasms was among 0.589 6-0.985 1. The results from POPGENE analysis indicated that the average information index （I） of Shannon was 0.370 2, gene diveristy （H） of Nei was 0.232 1, and effective number of alleles （NE） was 1.369 2. Based on the analyses of cluster and principal components, the 96 Dracaena cambodiana Pierre ex Gagnep germplasms used in this study could be divided into 4 groups.
出处
《热带作物学报》
CSCD
2011年第8期1518-1522,共5页
Chinese Journal of Tropical Crops
基金
海南省自然科学基金(309013)
农业部热带作物种质资源利用重点开放实验室项目(KFKT-2009-01)
海南大学科研启动基金项目(kyqd1037),海南大学热带作物种质资源保护与开发利用教育部重点实验室项目(2010hckled-04),海南大学211工程项目“热带作物遗传育种与生态保育”(QNJS-2011-06)
关键词
海南龙血树
ISSR
种质资源
遗传多样性
Dracaena cambodiana Pierre ex Gagnep
ISSR
Accessions
Genetic diversity
