摘要
高压诱导蛋白质发生构象变化,引起蛋白质去折叠,并在某种条件下发生聚集,其结果是蛋白质原有的构造被破坏、发生变性。这些过程与蛋白质本身的结构密切相关。同时,折叠中间体结构的揭示对于研究蛋白质去折叠过程也具有重要意义。本文综述了高压诱导下多种谱学方法用于蛋白质构象变化的研究进展,主要包括高压红外光谱、高压荧光光谱及高压小角X射线扫描等多种技术手段在研究中蛋白质构象变化方面所能提供的重要信息,并对相关领域存在的问题及今后的发展作出展望。
Pressure has been used for investigating the unfolding of proteins and for revealing their folding pathways and intermediates,even aggregate states.Since pressure can stabilize partially unfolded states and molten globules of proteins,which plays an important role both in chemical and biological phenomena in the aqueous solution.Here,we review several spectral methods developed for the study of pressure-induced conformation changes of proteins in solution,focus on infrared spectroscopy,fluorescence spectroscopy,small-angle X-ray scattering and so on,which can offer conformational information of proteins in solution in detail.The developing trends in this field are discussed and the outlook of the research area is also provided.
出处
《化学进展》
SCIE
CAS
CSCD
北大核心
2011年第10期2003-2011,共9页
Progress in Chemistry
基金
国家自然科学基金项目(No.209730042
0473028)资助
关键词
压力
蛋白质
水溶液
光谱
构象变化
聚集
pressure
proteins
aqueous solution
spectroscopy
conformation change
aggregation
