摘要
目的检测温敏性壳聚糖水凝胶对脂肪来源干细胞(ADSCs)的生物学性能影响。方法提取大鼠ADSCs和制备温敏性壳聚糖水凝胶,体外检测在温敏性壳聚糖水凝胶携带下的ADSCs细胞活力、增殖情况、多向分化潜能等生物学性能。结果与正常培养条件下ADSCs相比,温敏性壳聚糖水凝胶携带下的ADSCs细胞活力良好、增殖能力正常,并仍能保持多向分化潜能。结论温敏性壳聚糖水凝胶材料具有良好的细胞相容性,有望作为可注射性心肌组织工程研究中良好的可注射性支架材料。
Objective To study the effect of temperature–responsive chitosan hydrogel on biological performance of adipose-derived stem cells（ADSC）.Methods After ADSC were isolated and temperature-responsive chitosan hydrogel was prepared,biological performances of ADSC,including their vitality,proliferation and multipotential differentiation,were detected in temperature–responsive chitosan hydrogel in vitro.Results ADSCs were successfully cultured and confirmed by flow cytometry.When cocultured with chitosan hydrogel,ADSCs could grow very well and retain the biological performance of stem cells.After directional induction,they were able to further differentiate into cardiomyocyte-like cells,as well as adipogenic and osteogenic lineages.Conclusion Temperature–responsive chitosan hydrogel has a good biocompatibility,and can thus be used as an injectable bypass material in cardiac muscle engineering.
出处
《军医进修学院学报》
CAS
2011年第10期1059-1061,1072,共4页
Academic Journal of Pla Postgraduate Medical School
关键词
脂肪来源干细胞
壳聚糖水凝胶
生物学性能
adipose-derived stem cells
chitosan hydrogel
biological properties
