摘要
采用高效液相色谱法测定姜黄中主要姜黄素类化合物,色谱柱为C18,流动相为乙腈和0.5%冰醋酸,采样梯度洗脱,检测波长为425 nm。结果表明,姜黄样品中姜黄素类化合物的加样回收率分别为99.87%,99.91%,99.51%,RSD分别为0.75%,1.217%,1.06%。本方法对测定姜黄中主要的姜黄素类化合物快速简单,重复性好,为植物提取行业的含量测定提供依据。
The curcuminoids content in Curcuma longa L. was determined by HPLC with gradient elution with vensil XBP C18 column（4.6 nm×150 nm,5μm）, mobile phase of acetonitrile and 0.5% acetic acid. The detection wavelength was 425 nm. Results showed curcumin turmeric sample compounds recoveries were 99.87 %, 99.91%, 99.51%, RSD were 0.75 %, 1. 217 %, 1.06%. The method of determination of the major turmeric curcuminoids compounds was fast, simple and reproducible for the determination of plant extracts and provided the basis for the industry.
出处
《应用化工》
CAS
CSCD
2011年第9期1664-1665,1668,共3页
Applied Chemical Industry
