摘要
目的探索HO-1对肝脏缺血再灌注损伤中肥大细胞脱颗粒的影响。方法将20只SD大鼠随机分成4组：假手术组（Sham组），缺血再灌注损伤组（I／RI组），HO-1诱导剂钴原卟啉组（CoPP组，术前24h给予CoPP，5mg／kg）及HO-1抑制剂锌原卟啉组（ZnPP组，术前24h给予ZnPP，20mg／kg）。建立大鼠缺血再灌注损伤模型，各组于再灌注后2h收集标本。RT-PCR检测肝脏组织HO．1mRNA表达，Western blot检测肝脏组织HO-1蛋白表达；测定血清中ALT、AST水平；肝脏组织甲苯胺蓝染色检测肥大细胞脱颗粒数量，HE染色评价肝脏组织损伤情况。结果与Sham组相比，I／RI组、CoPP组、ZnPP组大鼠组织HO-1RNA和蛋白表达增加，血清ALT、AST水平升高，肥大细胞脱颗粒数量增多，肝脏细胞损伤加重。CoPP组与I／RI组相比，HO-1mRNA和蛋白表达增加，血清ALT、AST水平减低，肥大细胞脱颗粒数量减少，肝细胞损伤减轻。ZnPP组与I／RI组相比，HO-1mRNA和蛋白表达减少，血清ALT、AST水平升高，肥大细胞脱颗粒数量增多、肝细胞损伤严重。组间比较差异具有统计学意义（P〈0．05）。结论HO-1过表达能减轻肝脏I／RI，其机制可能与抑制肝脏组织中肥大细胞脱颗粒有关。
Objective To investigate the effects of HO-1 on mast ceils degranulation during liver ischemia./reperfusion injury. Methods SD rats （ n = 20） were randomly divided into 4 groups ： （ 1 ） sham operation group, （2） ischemia/reperfusion injury group （I/RI group）, （3） CoPP group, CoPP was given before 24 h（5 mg/kg） , （4） ZnPP group, ZnPP was given before 24 h （20 mg/kg）. The rat model of liver ischemia/reperfusion was built. Samples were collected after 2 hours reperfusion. HO-1 mRNA expression was assessed by RT-PCR. The expression of HO-lprotein was assessed by Western blot. Serum ALT, AST levels were determined. The amount of degranulated mast cells were detected by toluidine blue staining. Liver injuries were evaluated by HE staining. Results Compared to sham group, liver HO-1 mRNA and protein expression, ALT, AST levels, the number of degranulated mast cells significantly increased in I/RI group, CoPP group and ZnPP group. Moreover, liver injuries were more serious. Compared to I/RI group, there were increased HO-1mRNA and protein expression, reduced ALT, AST levels and the number of degranulated mast cells, also reduced liver injuries were found in CoPP group. Compared to I/RI group, the expression of HO-lmRNA and protein was down-regulated, ALT, AST levels elevated and the number of degranulated mast cells increased accompanying more serious liver injuries in ZnPP group. Conclusions HO-1 overexpression reduces liver ischemia/reperfusion injury, possibly by inhibiting mast cells degranulation in liver tissues.
出处
《中华普通外科杂志》
CSCD
北大核心
2011年第9期766-769,共4页
Chinese Journal of General Surgery
基金
国家自然科学基金资助项目（30600598）
上海市科学技术委员会医学重点基金资助项目（09411952500）
上海市科学技术委员会启明星后基金资助项目（10QH1401800）
上海市教育委员会“曙光计划”基金资助项目（IOSG20）
