摘要
利用钙离子选择微电极和普通微电极技术,观察了酸化蜍离体胃窦粘膜时粘膜细胞外Ca^(2+)活度和粘膜上皮细胞层与粘膜肌层之间电位差(以下简称跨粘膜电位)的变化及生长抑素、甲硫-脑啡肽和雨蛙肽对酸化粘膜效应的影响.实验结果表明,酸化胃窦粘膜引致细胞外Ca^(2+)活度和跨粘膜电位的下降.生长抑素阻止酸化粘膜效应而雨蛙肽则促进此效应,甲硫-脑啡肽无明显影响.结果提示生长抑素的细胞保护作用很可能与其抑制由酸刺激引起的细胞外Ca^(2+)活度降低作用有关.
To observe the changes in extracellular Ca^(2+) activity and mucosal membrane potential of toad antral mucosa stimulated by pH,and the effects of somatostatin, Met-enkephaline and caerulein on the changes, the experiments were performed with calcium ion selective microelectrode and common microelectrode techniques. The results obtained were as follows. The extracellular Ca^(2+) activity and mucosal membrane potential were decreased dose-responsively by acidification of the antral mucosa membrane. The decrease in extracellular Ca^(2+) activity was inhibited by pretreatment of the mucosa membrane with somatostatin (6.0×10^(-8) mol/L),while it was facilitated by caerulein(6. 0×10^(-8)mol/L). But it was not affected by pretreatment with Met-enkephaline(2. 35×10^(-5)mol/L). The results suggest that cytoprotection effect of somatostatin is probably related with its inhibitory action for decreased extracellular Ca^(2+) activity induced by pH stimmulation.
出处
《延边医学院学报》
CAS
1990年第3期166-170,共5页
Journal of Medical Science Yanbian University
基金
国家自然科学基金
吉林省卫生厅科研费资助课题
关键词
胃粘膜
钙
电位
肠肽
calcium
hydrogen ion concentration
mucous
membrane
potentiometry
enkephaline
somatostatin
caerulein
