摘要
目的通过检测结核分枝杆菌γ-干扰素酶联免疫斑点试验的结果,探讨其在菌阴肺结核诊断及鉴别诊断中的意义。方法将研究对象分为菌阳肺结核、菌阴肺结核、肺部肿瘤、肺炎4组。所有患者分别进行结核分枝杆菌γ-干扰素酶联免疫斑点试验、结核抗体三项测定及结核菌素试验。结果菌阳肺结核、菌阴肺结核、肺部肿瘤、肺炎4组患者PPD试验阳性率为84.8%、83.3%、28.6%、33.3%;结核抗体试验的阳性率为84.8%、66.6%、28.6%、33.3%;ELISPOT试验阳性率为93.9%、93.3%、7.1%、3.3%。结论结核杆菌感染T细胞酶联免疫斑点试验在肺结核诊断,可能优于结核菌素试验及结核抗体测定,对于菌阴肺结核的诊断与鉴别诊断具有临床意义。
Objective Through testing the mycobacterium tuberculosis γ-interferon ELISPOT results,this purpose discusses the meaning of diagnosis and differential diagnosis of Smear Negative Tuberculosis.Methods The research objects were divided into sputum positive pulmonary tuberculosis,smear negative pulmonary tuberculosis,lung tumor and pneumonia 4 groups.All of the patients were made mycobacterium tuberculosis gamma interferon ELISPOT test,tuberculosis antibody three tuberculin experiment and PPD test.Results The positive rates of PPD test in patients of sputum positive pulmonary tuberculosis,smear and culture negative pulmonary tuberculosis,lung cancer and pneumonia were 84.8%,83.3%,28.6%,33.3% respetively;tuberculosis antibody test positive rates were 84.8%,66.6%,28.6%,33.3% respectively;ELISPOT test was positive in 93.9%,93.3%,7.1%,3.3% respectively.Conclusion Mycobacterium tuberculosis infection in T cell ELISPOT test experience in the diagnosis of pulmonary tuberculosis and tuberculosis may be better than the tuberculin skin test and antibody test for the diagnosis and differential diagnosis of Smear Negative Tuberculosis have clinical significance.
出处
《临床肺科杂志》
2011年第11期1723-1724,共2页
Journal of Clinical Pulmonary Medicine
关键词
Γ-干扰素
结核分枝杆菌
酶联免疫斑点试验
γ-interferon
Mycobacterium tuberculosis
enzyme-linked immunosorbent spot test
