摘要
背景:研究发现肠神经胶质细胞(EGC)和环氧合酶-2(COX-2)参与了肠道炎症的发生、发展过程。目的:探讨神经胶质细胞特异性标记物胶质纤维酸性蛋白(GFAP)和COX-2在中重度活动期溃疡性结肠炎(UC)病变部位活检标本中的表达及其临床意义。方法:中重度活动期UC、腹泻型肠易激综合征(IBS-D)和正常对照者各30例纳入研究,以免疫组化方法检测活检标本中的GFAP、COX-2表达。结果:UC组GFAP强阳性表达率和免疫组化评分均低于IBS-D组和正常对照组,其中中度UC显著高于重度UC(P<0.05);UC组COX-2强阳性表达率和免疫组化评分均高于IBS-D组和正常对照组,其中中度UC显著低于重度UC(P<0.05)。UC病变累及广泛结肠者COX-2强阳性表达率显著高于病变仅累及部分肠段者(P<0.05),GFAP表达与UC病变范围无关。结论:活动期UC GFAP表达强度降低,COX-2表达强度增高,两者均与UC严重程度相关。
Background：It has been reported that enteric glial cells（EGC） and cyclooxygenase-2（COX-2） are involved in the process of inflammation in intestine.Aims：To investigate the expressions and clinical significance of glial fibrillary acidic protein（GFAP）,a specific marker of glial cells,and COX-2 in biopsy specimens of inflamed mucosa of moderate to severe active ulcerative colitis（UC）.Methods：Expressions of GFAP and COX-2 in biopsy specimens of 30 cases of moderate to severe active UC,30 cases of irritable bowel syndrome with diarrhea（IBS-D） and 30 cases of healthy subjects were determined by immunohistochemistry（IHC）.Results：The high-intensity expression rate and IHC score of GFAP in UC group were lower than those in IBS-D group and normal control group,and were higher in moderate UC than in severe UC（P0.05）.The high-intensity expression rate and IHC score of COX-2 in UC group were higher than those in IBS-D group and normal control group,and were lower in moderate UC than in severe UC（P0.05）.The high-intensity expression rate of COX-2 increased significantly in pan-colonic UC（P0.05）,while the expression of GFAP was not correlated with disease extent.Conclusions：Expression intensity of GFAP is decreased and that of COX-2 is increased in active UC. Both are correlated with the severity of UC.
出处
《胃肠病学》
2011年第8期469-472,共4页
Chinese Journal of Gastroenterology
