摘要
目的 建立莲草口服液中野黄芩苷的含量测定方法.方法 采用kromasil C18（250mm×4.6mm,5μm）色谱柱,以甲醇-水-醋酸（35∶61∶4）为流动相,检测波长为335nm.结果 野黄芩苷进样量在0.2~1.0μg范围内与峰面积线性关系良好（r=0.9999）（n=5）,平均回收率为100.2%,RSD=1.00%（n=6）.结论 该方法快速准确,重现性好,操作简便,可以作为莲草口服液质量控制标准.
Objective To develop a method of determining the content of scutellarin in Liancao Oral Liquid by HPLC. Methods Kromasil C18 column （250mm×4.6mm,5μm） was used. The mobile phase consisted of methanol-water-acetic acid （35：61：4）. The detection wavelength was at 335nm. Results There was a good linearity for scutellarin in the range of 0.2-1.0μg（r=0.9999）（n=5）. The average recovery rate was 100.2%, RSD=1.00%（n= 6）. Conclusion The method is simple, effective with good reproducibility, which can be used for the quality control of Liancao Oral Liquid.
作者
王青博
WANG Qing-bo（Furen Pharmaceutical Group Co., Zhengzhou 450003, China）
出处
《医学信息》
2011年第19期6419-6420,共2页
Journal of Medical Information
