摘要
目的:比较5种药用菊花试管苗在离体条件下对羟脯氨酸的耐受性,为药用菊花引种栽培和育种提供依据。方法:以5种药用菊花试管苗为材料,接种于添加不同浓度羟脯氨酸的培养基中培养,测定植株叶片内游离脯氨酸的含量,并统计植株的受害指数和存活率。结果和结论:在高浓度羟脯氨酸胁迫下,亳菊和怀菊试管苗受害指数较低,成活率相对较高,叶片内游离脯氨酸含量高,具有较强的HYP耐受性。滁菊和贡菊植株存活率低于亳菊和怀菊,表现为中等的HYP耐受性。杭菊试管苗存活率最低,HYP耐受性最差。
Objective： To investigate hydroxyproline tolerance of Chrysanthemum morifolium plantlets included ＂ Boju＂, ＂Huaiju＂ , ＂Chuju＂ , ＂Gongju＂ and ＂Hangju＂ ,and provide references basis for excellent cultivar and breeding of Ch. morifolium. Method： Plantlets in vitro from five kinds of Ch. morifolium were inoculated on medium added with different concentrations of hydroxyproline. Free proline in leaves from plantlets was determined, then the damage index and survival rate were compared. Result and Conclusion： The results showed that hydroxyproline tolerance of ＂Boju＂ and ＂ Huaiju＂ were superior, the survival rates and free proline of them were higher, but the damage index was inferior. The hydroxyproline tolerance of ＂ Hangju＂ was the worst, and the survival rate was minimum. The survival rate of ＂Chuju＂ and ＂ Gongju＂ was between ＂Boju＂ and ＂Hangju＂, and the hydroxyproline tol- erance of them was also medium.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第19期2625-2628,共4页
China Journal of Chinese Materia Medica
基金
安徽省自然科学基金项目(090413252)
安徽高校省级自然科学研究重点项目(KJ2009A160)
关键词
药用菊花
离体培养
胁迫筛选
羟脯氨酸
Chrysanthemum morifolium
culture in vitro
stress and selection
hydroxyproline
