摘要
采用SRAP标记对湖北省油茶5个居群的遗传多样性进行了研究.用11对引物对48个个体进行扩增,获得清晰的扩增条带64条,其中多态性条带60条,占93.75%;各材料间的遗传相似性系数(GS)在0.469～0.906之间,平均GS值为0.653;居群平均有效等位基因数(Ne)、平均Nei′s遗传多样性(H)、平均Shannon′s信息指数(I)分别为1.516 1、0.274 8、0.389 9,其结果表明供试油茶具有较为丰富的遗传多样性.聚类分析将居群分为2大类群,在分子水平上显示了其亲缘关系,为今后油茶育种亲本的选配提供理论依据.
Genetic diversity within and among five populations of Camellia oleifera from Hubei Province was revealed by SRAP marker. A total number of 64 discernable fragments were amplified from the 48 samples with 11 primers, 60 of which were polymorphic, and their polymorphism frequency was 93.75%. The genetic similarity coefficient of the tested accessions ranged from 0. 469 to 0. 906, and the average value was 0. 653; the average number of effective alleles per loci, Nei＇s gene diversity, Shannon＇s information index was 1. 5161, 0. 2748, 0. 3899, respectively. These results suggest that there was rich genetic diversity among Camellia oleifera tested. The tested populations were divided into two clusters, the genetic relationship revealed by molecular marker would be useful for selecting the parents in Camellia oleifera breeding programs.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第3期477-479,484,共4页
Journal of Central China Normal University：Natural Sciences
基金
湖北省教育厅项目(B20094001)
武汉市教育局项目(2008K080)
关键词
湖北省
油茶
遗传多样性
SRAP
Hubei Province
Camellia oleifera
genetic diversity
SRAP
