摘要
通过测定草鱼（Ctenopharyngodon idella）食用鱼不同颜色肝脏的水分和粗脂肪含量,并运用冰冻组织切片油红O染色技术对肝细胞进行染色,观察肝细胞脂肪滴的变化,利用Image-pro plus 6.0软件测定其阳性面积率,判断不同颜色肝脏的草鱼食用鱼脂肪肝程度.结果表明：与草鱼正常肝相比,花肝、发白肝和发黄肝的水分含量分别降低6.30%、10.01%和14.17%（P〈0.05）,粗脂肪含量分别升高198.25%、331.00%和485.59%（P〈0.05）;参照人肝细胞脂肪变性级别的判断标准,正常肝、花肝、发白肝和发黄肝的级别分别评为1.10±0.30、2.30±0.20、3.40±0.50和4.00±0.20;经Image-pro plus6.0软件分析,花肝、发白肝和发黄肝阳性面积率分别是正常肝的3.92、6.31和7.32倍;以上指标经回归分析得到的回归方程为：肝脏粗脂肪含量（Y）与水分（X）：Y=-1.089 9X＋82.035 7,R2=0.989（P〈0.01）;阳性面积率（Y）与粗脂肪含量（X）：Y=0.970 2X-0.209 0,R2=0.973（P〈0.05）;粗脂肪含量（Y）与肝细胞脂肪变性级别（X）：Y=3.662 4X-1.788 6,R2=0.985（P〈0.01）;阳性面积率（Y）与肝细胞脂肪变性级别（X）：Y=3.622 4X-2.130 6,R2=0.996（P〈0.01）.结果证实,冰冻组织切片油红O染色技术对鱼类脂肪肝的判断具有较好的参考价值,结合阳性面积率与肝脏粗脂肪含量、肝细胞脂肪变性级别具有显著的正相关,本试验建议将草鱼肝细胞阳性面积率〉2%定义为脂肪肝,其级别评定标准：2%~7%为轻度脂肪肝,7%~12%为中度脂肪肝,〉12%为重度脂肪肝.
The degree of fatty liver in different color livers for edible grass carp（Ctenopharyngodon idella） was discussed.Liver moisture was determined by freeze-drying method,and liver crude fat was measured gravimetrically after ether extract using Soxhlet method.The hepatocyte lipid droplets were observed by light microscopy in oil red O staining for frozen sections.Based on the criterion of human＇s fatty liver,the degree of fatty liver in edible grass carp was then determined by the positive area ratio.Compared with normal livers of grass carp,the moisture contents of motley livers,blushing livers and yellowing livers were decreased by 6.30%,10.01% and 14.17%（P〈0.05）,respectively;the crude fat contents were increased by 198.25%,331.00% and 485.59%（P〈0.05）,respectively;the number of hepatocyte lipid droplets were significantly increased with the peak in yellowing livers,and the positive area ratios were 3.92,6.31 and 7.32 times of the normal liver（P〈0.05）,respectively.The degree of hepatocyte fatty degeneration in normal liver,motley liver,blushing liver and yellowing liver was 1.10±0.30,2.30±0.20,3.40±0.50 and 4.00±0.20,respectively.After doing linear regression analysis,liver fat contents（Y） were negatively correlated with its moisture contents（X）： Y=-1.089 9X＋82.035 7,R2=0.989（P〈0.01）;the positive area ratio（Y） were positively correlated with its crude fat content（X）： Y=0.970 2X-0.209 0,R2=0.973（P〈0.05）;the crude fat content（Y） were positively correlated with the degree of hepatocyte fatty degeneration（X）： Y=3.662 4X-1.788 6,R2=0.985（P〈0.01）;the positive area ratios（Y） were also positively correlated with the degree of hepatocyte fatty degeneration（X）： Y=3.622 4X-2.130 6,R2=0.996（P〈0.01）.These results confirm that the oil red O staining method for frozen sections is suitable for evaluating the degree of fish fatty liver.As a result,according to the positive correlation between the positive area ratio and the crude fat content or the degree of hepatocyte fatty degeneration,it is suggested that the liver in grass carp can be defined as fatty liver when its positive area ratio exceeds 2%.Correspondingly,the degree of fatty liver can be defined as mild when its positive area ratio ranges from 2% to 7%,as medium when ranging from 7% to 12%,and as serious when exceeding 12%.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2011年第5期557-564,共8页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
2007年公益性行业(农业)科研专项经费资助项目(nyhyzx07-044)
"罗非鱼现代产业技术体系建设"资助项目(nycytx-48-9)
关键词
脂肪肝
油红O染色法
阳性面积率
草鱼
fatty liver
oil red O staining
positive area ratio
grass carp
