摘要
为探讨绵羊透明带对其精子顶体反应的诱导作用,试验分为3组:①不经获能处理的精子与透明带共孵育,②精子经获能处理后与透明带共孵育,③不经获能处理的精子与透明带共孵育,然后添加IA23187,来观察透明带对精子顶体反应的诱导效果。结果显示获能与否并不影响透明带对精子顶体反应的诱导效果,添加IA23187后,顶体反应升高,但差异不显著(P>0.05)。说明透明带可诱导绵羊精子(无论获能与否)发生顶体反应,IA23187不能提高透明带对顶体反应的诱导作用。
To find out the effects of ovine sperm acrosome reaction induced by zone pellucid,three experiments were designed：first,co-incubation non-capacited sperm with zone pellucid;second,co-incubation capacitation sperm with zone pellucid;third,adding with calcium ionophore A23187 after co-incubation non-capacited sperm with zone pellucid.The results showed that the rates of acrosome reaction induced by zone pellucid are not significant difference whether the spermatozoa were capacited,IA23187 don ＇ t cooperate with zone pellucide to improve the rate of acrosome reaction.
出处
《中国农学通报》
CSCD
北大核心
2011年第23期29-31,共3页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金资助项目"MUC1协调GLYCAM1和ISG15调控绵羊子宫容受态机制的研究"(30972228)
关键词
绵羊精子
获能
顶体反应
透明带
钙离子载体
ovine sperm
capacitation
acrosome reaction
zone pellucide
calcium ionophore
