摘要
研究电子束辐照对花粉萌发率的影响,为电子束辐射诱变育种提供实验基础。对‘京糯28’玉米花粉和陆地棉花粉进行不同剂量的电子束辐照,在液体培养基中对辐照的花粉进行培养,观察并统计二者的花粉萌发率。玉米‘京糯28’花粉在200～600Gy辐射剂量范围内花粉萌发率均高于对照组,其中以600Gy辐射剂量的萌发率最高;而陆地棉在300～1000Gy辐射剂量范围内花粉的萌发率均低于对照组,但在50h后对其花粉进行培养时,各辐照组的花粉萌发率均高于对照组。电子束辐照剂量在200～600Gy范围内对‘京糯28’玉米花粉萌发率有促进作用,在300～1000Gy范围内对陆地棉花粉萌发率有不同程度的抑制作用,但在一定程度上延长了陆地棉花粉生活力。
The aim was to study the effects of electron beam irradiation on pollen germination rate and provide the base for mutation breeding of electron beam irradiation.The maize pollen of ＇ Beijing-waxy 28 ＇ and pollen of Gossypium hirsutum L.were exposed in electron beam with different dose,the irradiated pollens were cultivated in a liquid medium,and then the pollen germination rates were observed and counted.The pollen germination rate of ＇ Beijing-waxy 28 ＇ was higher than that of the control group in 200-600 Gy radiation dose,with the highest germination rate at 600 Gy radiation dose.While the pollen germination rate of Gossypium hirsutum L.was lower than that of the control group in 300-1000 Gy radiation dose,but after the 50 h,the pollen germination rate was higher than that of the control groups.Electron beam radiation doses in the range of 200-600 Gy could facilitate the pollen germination rate of ＇ Beijing-waxy 28 ＇,and electron beam radiation doses in the range of 300-1000 Gy could inhibit the pollen germination rate of Gossypium hirsutum L.,but it could extend the activity of the Gossypium hirsutum L.pollen in a certain degree.
出处
《中国农学通报》
CSCD
北大核心
2011年第24期164-168,共5页
Chinese Agricultural Science Bulletin
基金
射线束技术与材料改性教育部重点开放实验室基金课题"离子注入植物花粉的生物学效应的研究"(201006)
关键词
电子束辐射
花粉萌发率
玉米
陆地棉
electron beam radiation
pollen germination rate
maize
Gossypium hirsutum L.
