摘要
利用SRAP标记对黑龙江地区采集的30株野生黑木耳菌株进行遗传多样性分析,利用8对扩增条带清晰、多态性丰富、稳定性较好的引物对30个供试菌株进行SRAP扩增,共得到157条重复性良好的DNA条带,其中多态性条带122条,占77.71%;使用UPGMA法构建进化树,30个菌株被分为6组。结果表明,黑龙江省野生黑木耳菌株间存在着丰富的遗传多样性。本研究为将来杂交育种亲本的选择提供很好的理论依据。
30 wild Auricularia auricula strains were collected from Heilongjiang Province and analyzed by using sequence-related amplified polymorphism（SRAP） markers.8 SRAP primer combinations amplified 157 fragments of which 122（77.71%） were polymorphic.Phylogenetic tree was constructed by using the Unweighted Pair-group Method with Arithmetic Averages（UPGMA） method.30 strains distributed into six groups.The clustering analysis showed that there was a high level of genetic diversity among A.auricula.This study would provide good reference for parent selection in further cross-breeding programs.
出处
《中国农学通报》
CSCD
北大核心
2011年第22期182-186,共5页
Chinese Agricultural Science Bulletin
基金
国家"十一五"科技支撑项目(2008BADA1B01)
东北农业大学创新团队发展计划基金资助(CXT003-2-1)
