摘要
目的:探讨过度运动对大鼠肾组织及肾损伤敏感标志物的影响,及人参总皂苷的防治作用。方法:SD大鼠随机分为对照组(CTL)、过度运动组(M2、M24两个亚组),人参总皂苷组(G2、G24两个亚组),每组7只。采用大鼠运动跑台建立过度运动致大鼠急性肾损伤模型,人参总皂苷组于运动前1周给予人参总皂苷100mg·kg-1·d-1灌胃,模型组和对照组给予等体积蒸馏水灌胃,分别于运动后2h,24h检测尿蛋白、中性粒细胞明胶酶载脂蛋白(neutrophil gelatinase-associated lipocalin,NGAL)、N-乙酰β-D氨基葡萄糖苷酶(NAG酶),腹主动脉采血检测肾功能变化,检测血浆及肾组织中血管紧张素Ⅱ(AngⅡ)浓度、SOD活性和MDA含量;光镜下观察肾组织病理改变。结果:过度运动可引起大鼠尿蛋白、尿NAG酶和血尿素氮、血NGAL水平升高,血浆及肾组织中AngⅡ浓度升高;伴随血清及肾组织中SOD活性降低、MDA含量升高,病理学光镜可见少量肾小管上皮细胞肿胀和脱落。人参总皂苷可明显降低大鼠过度运动后尿素氮水平,减少过度运动后蛋白尿和尿NAG酶排泄,抑制运动后肾组织AngⅡ升高,升高血清及肾组织中SOD活性,降低MDA含量(P<0.05),改善运动后肾脏病理损伤。结论:过度运动可引起尿蛋白及尿酶升高,及肾小管损伤;人参总皂苷对大鼠过度运动后AKI具有预防保护作用,其机制可能与抗氧化应激及减少运动后肾组织AngⅡ含量有关。
Objective：We investigate the changes of some sensitive biomarkers in rats undertaking excessive-exercise,and the effects of Ginsenoside （GS） which was a product from a plant herb-Ginsen.Methods：Male Sprague-Dawley rats divided into five groups： control group（CTL）,over-exercise group（ two sub-group： M2 and M24）;Ginsenoside group （ two sub-group： G2 and G24）.The animal model of excessive-exercise induced AKI was established by exhaustively running on the treadmill according protocol.We administrated with GS （100g/kg.d） intragastric ahead the G2 and G24 rats took exhausting running.We collected urine 2h or 24h after excessive-exercise,and measured their albumin,neutrophil gelatinase-associated lipocalin （NGAL） and N-acetyl--d-glucosaminidase（NAG）.We collected blood to test the renal function （BUN,serum creatinine）,superoxide dismutase（SOD） and malondialdehyde （MDA）.The blood and kidney Angiotensin Ⅱ（AngⅡ） were measured by radioimmunoassay （RIA）.We observed the renal pathology by Microscope after HE staining.Results：Over-exercise induced the urine albumin and NAG excretion increasing,the BUN and blood NGAL increased too.Compared with the blood and kidney AngⅡincreased,the serum and kidney tissue SOD decreased,but MDA increased at the same time.We observed a part of the renal tubular epithelial cells swelling and ablating.After treatment by GS,the excretion of urine albumin and NAG reduced,BUN and NGAL in blood decreased.GS decreased the kidney Angiotensin Ⅱand MDA level,but increased the SOD level in blood or kidney tissue,thus,ameliorate the tubular injury.Conclusion：Ginsenoside protect the tubular injury induced by the over-exercise in rats,which mechanism might reduce the Ang Ⅱ and anti-oxidative stress in the kidney.
出处
《中国中西医结合肾病杂志》
2011年第9期769-772,848,共5页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
南京军区医药卫生基金重点项目(No.2008132002)
